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Sequence finishing and mapping of Drosophila melanogaster heterochromatin.

Genome sequences for most metazoans and plants are incomplete because of the presence of repeated DNA in the heterochromatin. The heterochromatic regions of Drosophila melanogaster contain 20 million bases (Mb) of sequence amenable to mapping, sequence assembly, and finishing. We describe the generation of 15 Mb of finished or improved heterochromatic sequence with the use of available clone resources and assembly methods. We also constructed a bacterial artificial chromosome-based physical map that spans 13 Mb of the pericentromeric heterochromatin and a cytogenetic map that positions 11 Mb in specific chromosomal locations. We have approached a complete assembly and mapping of the nonsatellite component of Drosophila heterochromatin. The strategy we describe is also applicable to generating substantially more information about heterochromatin in other species, including humans.

Pubmed ID: 17569867

Authors

  • Hoskins RA
  • Carlson JW
  • Kennedy C
  • Acevedo D
  • Evans-Holm M
  • Frise E
  • Wan KH
  • Park S
  • Mendez-Lago M
  • Rossi F
  • Villasante A
  • Dimitri P
  • Karpen GH
  • Celniker SE

Journal

Science (New York, N.Y.)

Publication Data

June 15, 2007

Associated Grants

  • Agency: NHGRI NIH HHS, Id: P50-HG00750
  • Agency: NIGMS NIH HHS, Id: R01 GM066272
  • Agency: NHGRI NIH HHS, Id: R01 HG000747
  • Agency: NHGRI NIH HHS, Id: R01 HG000747-16
  • Agency: NHGRI NIH HHS, Id: R01 HG00747

Mesh Terms

  • Animals
  • Chromosome Mapping
  • Chromosomes, Artificial, Bacterial
  • Contig Mapping
  • Drosophila melanogaster
  • Genome
  • Heterochromatin
  • In Situ Hybridization, Fluorescence
  • Physical Chromosome Mapping
  • Sequence Analysis, DNA