Ribosome synthesis in eukaryotes requires a multitude of trans-acting factors. These factors act at many steps as the pre-ribosomal particles travel from the nucleolus to the cytoplasm. In contrast to the well-studied trans-acting factors, little is known about the contribution of the ribosomal proteins to ribosome biogenesis. Herein, we have analysed the role of ribosomal protein Rpl3p in 60S ribosomal subunit biogenesis. In vivo depletion of Rpl3p results in a deficit in 60S ribosomal subunits and the appearance of half-mer polysomes. This phenotype is likely due to the instability of early and intermediate pre-ribosomal particles, as evidenced by the low steady-state levels of 27SA(3), 27SB(S) and 7S(L/S) precursors. Furthermore, depletion of Rpl3p impairs the nucleocytoplasmic export of pre-60S ribosomal particles. Interestingly, flow cytometry analysis indicates that Rpl3p-depleted cells arrest in the G1 phase. Altogether, we suggest that upon depletion of Rpl3p, early assembly of 60S ribosomal subunits is aborted and subsequent steps during their maturation and export prevented.
Pubmed ID: 17569673 RIS Download
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A program of regional geochemical and geophysical surveys designed to attract the mineral exploration industry to an under-explored region of British Columbia between Williams Lake and Mackenize. The QUEST Project is focused on the Quesnel Terrane, which has good potential for copper and gold porphyry deposits, but in this region is covered by a thick layer of sand and gravel left behind by glaciers. Geoscience BC's QUEST Project included two airborne geophysical surveys: an airborne electromagnetic (EM) survey and an airborne gravity survey, which give insight into the conductivity and density of the rocks. The results of the two airborne surveys are an important tool for industry looking to understand the geology in the QUEST Project area. The QUEST Project also included the reanalysis of almost 5000 archived regional geochemical samples from parts of NTS sheets 93A, B, G, H, K and N, and the collection of 2200 new geochemical samples north and west of Prince George. This new geochemical data is helping industry to identify new exploration targets. Subsequent follow-up work has included analysis and inversion of datasets and updating the regional bedrock geology map.
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