• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Regulation of dendritogenesis via a lipid-raft-associated Ca2+/calmodulin-dependent protein kinase CLICK-III/CaMKIgamma.

Ca(2+) signaling plays a central role in activity-dependent regulation of dendritic arborization, but key molecular mechanisms downstream of calcium elevation remain poorly understood. Here we show that the C-terminal region of the Ca(2+)/calmodulin-dependent protein kinase CLICK-III (CL3)/CaMKIgamma, a membrane-anchored CaMK, was uniquely modified by two sequential lipidification steps: prenylation followed by a kinase-activity-regulated palmitoylation. These modifications were essential for CL3 membrane anchoring and targeting into detergent-resistant lipid microdomains (or rafts) in the dendrites. We found that CL3 critically contributed to BDNF-stimulated dendritic growth. Raft insertion of CL3 specifically promoted dendritogenesis of cortical neurons by acting upstream of RacGEF STEF and Rac, both present in lipid rafts. Thus, CL3 may represent a key element in the Ca(2+)-dependent and lipid-raft-delineated switch that turns on extrinsic activity-regulated dendrite formation in developing cortical neurons.

Pubmed ID: 17553424

Authors

  • Takemoto-Kimura S
  • Ageta-Ishihara N
  • Nonaka M
  • Adachi-Morishima A
  • Mano T
  • Okamura M
  • Fujii H
  • Fuse T
  • Hoshino M
  • Suzuki S
  • Kojima M
  • Mishina M
  • Okuno H
  • Bito H

Journal

Neuron

Publication Data

June 7, 2007

Associated Grants

None

Mesh Terms

  • Animals
  • Brain-Derived Neurotrophic Factor
  • COS Cells
  • Calcium Signaling
  • Calcium-Calmodulin-Dependent Protein Kinase Type 1
  • Calcium-Calmodulin-Dependent Protein Kinases
  • Cell Differentiation
  • Cercopithecus aethiops
  • Cerebral Cortex
  • Dendrites
  • Glycosylphosphatidylinositols
  • Membrane Microdomains
  • Mice
  • Palmitic Acid
  • Protein Prenylation
  • Protein Processing, Post-Translational
  • Protein Structure, Tertiary
  • Rats
  • Rats, Sprague-Dawley
  • rac GTP-Binding Proteins