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Regulation of dendritogenesis via a lipid-raft-associated Ca2+/calmodulin-dependent protein kinase CLICK-III/CaMKIgamma.

Neuron | Jun 7, 2007

http://www.ncbi.nlm.nih.gov/pubmed/17553424

Ca(2+) signaling plays a central role in activity-dependent regulation of dendritic arborization, but key molecular mechanisms downstream of calcium elevation remain poorly understood. Here we show that the C-terminal region of the Ca(2+)/calmodulin-dependent protein kinase CLICK-III (CL3)/CaMKIgamma, a membrane-anchored CaMK, was uniquely modified by two sequential lipidification steps: prenylation followed by a kinase-activity-regulated palmitoylation. These modifications were essential for CL3 membrane anchoring and targeting into detergent-resistant lipid microdomains (or rafts) in the dendrites. We found that CL3 critically contributed to BDNF-stimulated dendritic growth. Raft insertion of CL3 specifically promoted dendritogenesis of cortical neurons by acting upstream of RacGEF STEF and Rac, both present in lipid rafts. Thus, CL3 may represent a key element in the Ca(2+)-dependent and lipid-raft-delineated switch that turns on extrinsic activity-regulated dendrite formation in developing cortical neurons.

Pubmed ID: 17553424 RIS Download

Mesh terms: Animals | Brain-Derived Neurotrophic Factor | COS Cells | Calcium Signaling | Calcium-Calmodulin-Dependent Protein Kinase Type 1 | Calcium-Calmodulin-Dependent Protein Kinases | Cell Differentiation | Cercopithecus aethiops | Cerebral Cortex | Dendrites | Glycosylphosphatidylinositols | Membrane Microdomains | Mice | Palmitic Acid | Protein Prenylation | Protein Processing, Post-Translational | Protein Structure, Tertiary | Rats | Rats, Sprague-Dawley | rac GTP-Binding Proteins

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