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Selective stimulation of astrocyte calcium in situ does not affect neuronal excitatory synaptic activity.

Astrocytes are considered the third component of the synapse, responding to neurotransmitter release from synaptic terminals and releasing gliotransmitters--including glutamate--in a Ca(2+)-dependent manner to affect neuronal synaptic activity. Many studies reporting astrocyte-driven neuronal activity have evoked astrocyte Ca(2+) increases by application of endogenous ligands that directly activate neuronal receptors, making astrocyte contribution to neuronal effect(s) difficult to determine. We have made transgenic mice that express a Gq-coupled receptor only in astrocytes to evoke astrocyte Ca(2+) increases using an agonist that does not bind endogenous receptors in brain. By recording from CA1 pyramidal cells in acute hippocampal slices from these mice, we demonstrate that widespread Ca(2+) elevations in 80%-90% of stratum radiatum astrocytes do not increase neuronal Ca(2+), produce neuronal slow inward currents, or affect excitatory synaptic activity. Our findings call into question the developing consensus that Ca(2+)-dependent glutamate release by astrocytes directly affects neuronal synaptic activity in situ.

Pubmed ID: 17521573

Authors

  • Fiacco TA
  • Agulhon C
  • Taves SR
  • Petravicz J
  • Casper KB
  • Dong X
  • Chen J
  • McCarthy KD

Journal

Neuron

Publication Data

May 24, 2007

Associated Grants

None

Mesh Terms

  • Animals
  • Animals, Newborn
  • Astrocytes
  • Calcium
  • Cell Communication
  • Drug Interactions
  • Excitatory Postsynaptic Potentials
  • GTP-Binding Protein alpha Subunits, Gq-G11
  • Green Fluorescent Proteins
  • Hippocampus
  • In Vitro Techniques
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Neurotransmitter Agents
  • Oligopeptides
  • Patch-Clamp Techniques
  • Phosphopyruvate Hydratase
  • Presynaptic Terminals
  • Pyramidal Cells
  • Receptors, G-Protein-Coupled
  • Synapses
  • Synaptic Transmission