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Positional stability of single double-strand breaks in mammalian cells.

Nature cell biology | Jun 1, 2007

http://www.ncbi.nlm.nih.gov/pubmed/17486118

Formation of cancerous translocations requires the illegitimate joining of chromosomes containing double-strand breaks (DSBs). It is unknown how broken chromosome ends find their translocation partners within the cell nucleus. Here, we have visualized and quantitatively analysed the dynamics of single DSBs in living mammalian cells. We demonstrate that broken ends are positionally stable and unable to roam the cell nucleus. Immobilization of broken chromosome ends requires the DNA-end binding protein Ku80, but is independent of DNA repair factors, H2AX, the MRN complex and the cohesion complex. DSBs preferentially undergo translocations with neighbouring chromosomes and loss of local positional constraint correlates with elevated genomic instability. These results support a contact-first model in which chromosome translocations predominantly form among spatially proximal DSBs.

Pubmed ID: 17486118 RIS Download

Mesh terms: Animals | Antigens, Nuclear | Cell Cycle Proteins | Cell Nucleus | Cell Transformation, Neoplastic | Chromosomal Proteins, Non-Histone | Chromosomes | DNA | DNA Breaks, Double-Stranded | DNA Damage | DNA Repair | DNA-Binding Proteins | Genomic Instability | Histones | Macromolecular Substances | Mice | NIH 3T3 Cells | Nuclear Proteins | Translocation, Genetic

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Associated grants

  • Agency: NIGMS NIH HHS, Id: GM68956
  • Agency: Intramural NIH HHS, Id: Z01 BC010309-09

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