• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Expression of the LIM-homeodomain protein Isl1 in the developing and mature mouse retina.

The mammalian retina is comprised of six major neuronal cell types and is subdivided into more morphological and physiological subtypes. The transcriptional machinery underlying these subtype fate choices is largely unknown. The LIM-homeodomain protein, Isl1, plays an essential role in central nervous system (CNS) differentiation but its relationship to retinal neurogenesis remains unknown. We report here its dynamic spatiotemporal expression in the mouse retina. Among bipolar interneurons, Isl1 expression commences at postnatal day (P)5 and is later restricted to ON-bipolar cells. The intensity of Isl1 expression is found to segregate the pool of ON-bipolar cells into rod and ON-cone bipolar cells with higher expression in rod bipolar cells. As bipolar cell development proceeds from P5-10 the colocalization of Isl1 and the pan-bipolar cell marker Chx10 reveals the organization of ON-center bipolar cell nuclei to the upper portion of the inner nuclear layer. Further, whereas Isl1 is predominantly a ganglion cell marker prior to embryonic day (E)15.5, at E15.5 and later its expression in nonganglion cells expands. We demonstrate that these Isl1-positive, nonganglion cells acquire the expression of amacrine cell markers embryonically, likely representing nascent cholinergic amacrine cells. Taken together, Isl1 is expressed during the maturation of and is later maintained in retinal ganglion cells and subtypes of amacrine and bipolar cells where it may function in the maintenance of these cells into adulthood.

Pubmed ID: 17480014

Authors

  • Elshatory Y
  • Deng M
  • Xie X
  • Gan L

Journal

The Journal of comparative neurology

Publication Data

July 1, 2007

Associated Grants

  • Agency: NEI NIH HHS, Id: EY013426
  • Agency: NEI NIH HHS, Id: R01 EY013426
  • Agency: NEI NIH HHS, Id: R01 EY013426-05
  • Agency: NINDS NIH HHS, Id: T32 NS07489

Mesh Terms

  • Amacrine Cells
  • Animals
  • Cell Differentiation
  • Gene Expression Regulation, Developmental
  • Homeodomain Proteins
  • Immunohistochemistry
  • LIM-Homeodomain Proteins
  • Mice
  • Mice, Inbred C57BL
  • Retina
  • Retinal Bipolar Cells
  • Retinal Ganglion Cells
  • Transcription Factors