A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis.
Dynamin 1 is a neuron-specific guanosine triphosphatase thought to be critically required for the fission reaction of synaptic vesicle endocytosis. Unexpectedly, mice lacking dynamin 1 were able to form functional synapses, even though their postnatal viability was limited. However, during spontaneous network activity, branched, tubular plasma membrane invaginations accumulated, capped by clathrin-coated pits, in synapses of dynamin 1-knockout mice. Synaptic vesicle endocytosis was severely impaired during strong exogenous stimulation but resumed efficiently when the stimulus was terminated. Thus, dynamin 1-independent mechanisms can support limited synaptic vesicle endocytosis, but dynamin 1 is needed during high levels of neuronal activity.
Pubmed ID: 17463283 RIS Download
Action Potentials | Animals | Cell Membrane | Clathrin-Coated Vesicles | Dynamin I | Dynamin II | Dynamin III | Electric Stimulation | Endocytosis | Excitatory Postsynaptic Potentials | Exocytosis | Inhibitory Postsynaptic Potentials | Mice | Mice, Knockout | Microscopy, Electron | Neurons | Patch-Clamp Techniques | Presynaptic Terminals | Synapses | Synaptic Transmission | Synaptic Vesicles