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A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis.

Dynamin 1 is a neuron-specific guanosine triphosphatase thought to be critically required for the fission reaction of synaptic vesicle endocytosis. Unexpectedly, mice lacking dynamin 1 were able to form functional synapses, even though their postnatal viability was limited. However, during spontaneous network activity, branched, tubular plasma membrane invaginations accumulated, capped by clathrin-coated pits, in synapses of dynamin 1-knockout mice. Synaptic vesicle endocytosis was severely impaired during strong exogenous stimulation but resumed efficiently when the stimulus was terminated. Thus, dynamin 1-independent mechanisms can support limited synaptic vesicle endocytosis, but dynamin 1 is needed during high levels of neuronal activity.

Pubmed ID: 17463283


  • Ferguson SM
  • Brasnjo G
  • Hayashi M
  • Wölfel M
  • Collesi C
  • Giovedi S
  • Raimondi A
  • Gong LW
  • Ariel P
  • Paradise S
  • O'toole E
  • Flavell R
  • Cremona O
  • Miesenböck G
  • Ryan TA
  • De Camilli P


Science (New York, N.Y.)

Publication Data

April 27, 2007

Associated Grants

  • Agency: NCI NIH HHS, Id: CA46128
  • Agency: Telethon, Id: D.061
  • Agency: NIDA NIH HHS, Id: DA018343
  • Agency: NIDA NIH HHS, Id: DA17297
  • Agency: NIDDK NIH HHS, Id: DK45735
  • Agency: NINDS NIH HHS, Id: NS036942
  • Agency: NINDS NIH HHS, Id: NS36251
  • Agency: NIDA NIH HHS, Id: P30 DA018343
  • Agency: NINDS NIH HHS, Id: R01 NS036942
  • Agency: NCRR NIH HHS, Id: RR-000592

Mesh Terms

  • Action Potentials
  • Animals
  • Cell Membrane
  • Clathrin-Coated Vesicles
  • Dynamin I
  • Dynamin II
  • Dynamin III
  • Electric Stimulation
  • Endocytosis
  • Excitatory Postsynaptic Potentials
  • Exocytosis
  • Inhibitory Postsynaptic Potentials
  • Mice
  • Mice, Knockout
  • Microscopy, Electron
  • Neurons
  • Patch-Clamp Techniques
  • Presynaptic Terminals
  • Synapses
  • Synaptic Transmission
  • Synaptic Vesicles