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Critical functions of N-glycans in L-selectin-mediated lymphocyte homing and recruitment.

Nature immunology | Apr 21, 2007

Lymphocyte homing is mediated by specific interaction between L-selectin on lymphocytes and the carbohydrate ligand 6-sulfo sialyl Lewis X on high endothelial venules. Here we generated mice lacking both core 1 extension and core 2 branching enzymes to assess the functions of O-glycan-borne L-selectin ligands in vivo. Mutant mice maintained robust lymphocyte homing, yet they lacked O-glycan L-selectin ligands. Biochemical analyses identified a class of N-glycans bearing the 6-sulfo sialyl Lewis X L-selectin ligand in high endothelial venules. These N-glycans supported the binding of L-selectin to high endothelial venules in vitro and contributed in vivo to O-glycan-independent lymphocyte homing in wild-type and mutant mice. Our results demonstrate the critical function of N-glycan-linked 6-sulfo sialyl Lewis X in L-selectin-dependent lymphocyte homing and recruitment.

Pubmed ID: 17334369 RIS Download

Mesh terms: Animals | Antigens, Surface | Cell Adhesion | Dermatitis, Contact | Endothelium, Lymphatic | L-Selectin | Lymph Nodes | Lymphocytes | Membrane Proteins | Mice | Mice, Knockout | Microscopy, Fluorescence | Oligosaccharides | Polysaccharides

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Associated grants

  • Agency: NIAID NIH HHS, Id: AI061663
  • Agency: NIAID NIH HHS, Id: AI069259
  • Agency: NIAMS NIH HHS, Id: AR42689
  • Agency: NCI NIH HHS, Id: CA48737
  • Agency: NIDDK NIH HHS, Id: DK48247
  • Agency: NCI NIH HHS, Id: P01 CA71932

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