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Reconstitution of DNA segregation driven by assembly of a prokaryotic actin homolog.

Multiple unrelated polymer systems have evolved to partition DNA molecules between daughter cells at division. To better understand polymer-driven DNA segregation, we reconstituted the three-component segregation system of the R1 plasmid from purified components. We found that the ParR/parC complex can construct a simple bipolar spindle by binding the ends of ParM filaments, inhibiting dynamic instability, and acting as a ratchet permitting incorporation of new monomers and riding on the elongating filament ends. Under steady-state conditions, the dynamic instability of unattached ParM filaments provides the energy required to drive DNA segregation.

Pubmed ID: 17332412


  • Garner EC
  • Campbell CS
  • Weibel DB
  • Mullins RD


Science (New York, N.Y.)

Publication Data

March 2, 2007

Associated Grants

  • Agency: NIGMS NIH HHS, Id: R01 GM061010
  • Agency: NIGMS NIH HHS, Id: R01 GM061010-07
  • Agency: NIGMS NIH HHS, Id: R01GM61010
  • Agency: NIGMS NIH HHS, Id: R01GM675287

Mesh Terms

  • Actins
  • Adenosine Triphosphate
  • Bacterial Proteins
  • Biopolymers
  • DNA Topoisomerase IV
  • DNA, Bacterial
  • Escherichia coli Proteins
  • Microspheres
  • Protein Binding
  • R Factors
  • Repressor Proteins