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Reconstitution of DNA segregation driven by assembly of a prokaryotic actin homolog.

Science (New York, N.Y.) | Mar 2, 2007

http://www.ncbi.nlm.nih.gov/pubmed/17332412

Multiple unrelated polymer systems have evolved to partition DNA molecules between daughter cells at division. To better understand polymer-driven DNA segregation, we reconstituted the three-component segregation system of the R1 plasmid from purified components. We found that the ParR/parC complex can construct a simple bipolar spindle by binding the ends of ParM filaments, inhibiting dynamic instability, and acting as a ratchet permitting incorporation of new monomers and riding on the elongating filament ends. Under steady-state conditions, the dynamic instability of unattached ParM filaments provides the energy required to drive DNA segregation.

Pubmed ID: 17332412 RIS Download

Mesh terms: Actins | Adenosine Triphosphate | Bacterial Proteins | Biopolymers | DNA Topoisomerase IV | DNA, Bacterial | Escherichia coli Proteins | Microspheres | Protein Binding | R Factors | Repressor Proteins

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