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qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data.

Genome biology | Apr 17, 2007

http://www.ncbi.nlm.nih.gov/pubmed/17291332

Although quantitative PCR (qPCR) is becoming the method of choice for expression profiling of selected genes, accurate and straightforward processing of the raw measurements remains a major hurdle. Here we outline advanced and universally applicable models for relative quantification and inter-run calibration with proper error propagation along the entire calculation track. These models and algorithms are implemented in qBase, a free program for the management and automated analysis of qPCR data.

Pubmed ID: 17291332 RIS Download

Mesh terms: Algorithms | Gene Expression Profiling | Models, Genetic | Polymerase Chain Reaction | Software