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qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data.

Although quantitative PCR (qPCR) is becoming the method of choice for expression profiling of selected genes, accurate and straightforward processing of the raw measurements remains a major hurdle. Here we outline advanced and universally applicable models for relative quantification and inter-run calibration with proper error propagation along the entire calculation track. These models and algorithms are implemented in qBase, a free program for the management and automated analysis of qPCR data.

Pubmed ID: 17291332

Authors

  • Hellemans J
  • Mortier G
  • De Paepe A
  • Speleman F
  • Vandesompele J

Journal

Genome biology

Publication Data

April 17, 2007

Associated Grants

None

Mesh Terms

  • Algorithms
  • Gene Expression Profiling
  • Models, Genetic
  • Polymerase Chain Reaction
  • Software