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Ubiquitous and uniform in vivo fluorescence in ROSA26-EGFP BAC transgenic mice.

Transplantation studies and cell lineage analyses require the ability to explicitly distinguish morphologically identical cells that have an identifiable marker indicating their origin in vivo. Several reporter mouse strains have been generated for such studies, but pancellular detection of the marker in all tissues has not been achieved. In this report, we describe the generation of transgenic mice that express enhanced green fluorescent protein (EGFP) under control of a 187 kb bacterial artificial chromosome (BAC) containing the murine ROSA26 locus, and show several advantages over existing EGFP reporter lines. It is demonstrated that EGFP is ubiquitously and reproducibly expressed from the murine BAC transgene in all organs and tissues analyzed, including the hematolymphoid compartment. Using this new reporter strain in hematopoietic cell transplantation studies, it is demonstrated that leukocytes in recipients maintain uniform transgene expression and are easily distinguished by flow cytometric analysis of live cells. The results suggest that the ROSA26 BAC is an efficient strategy for expressing complex transgene cassettes in vivo.

Pubmed ID: 17269129

Authors

  • Giel-Moloney M
  • Krause DS
  • Chen G
  • Van Etten RA
  • Leiter AB

Journal

Genesis (New York, N.Y. : 2000)

Publication Data

February 8, 2007

Associated Grants

  • Agency: NCI NIH HHS, Id: CA105043
  • Agency: NCI NIH HHS, Id: CA90576
  • Agency: NIDDK NIH HHS, Id: DK43673
  • Agency: NIDDK NIH HHS, Id: DK52870
  • Agency: NIDDK NIH HHS, Id: DK67166
  • Agency: NIDDK NIH HHS, Id: P30 DK034928
  • Agency: NIDDK NIH HHS, Id: P30 DK034928-239002
  • Agency: NIDDK NIH HHS, Id: P30-DK34928
  • Agency: NCI NIH HHS, Id: R01 CA090576
  • Agency: NCI NIH HHS, Id: R01 CA090576-07
  • Agency: NCI NIH HHS, Id: R01 CA105043
  • Agency: NCI NIH HHS, Id: R01 CA105043-03
  • Agency: NIDDK NIH HHS, Id: R01 DK067166
  • Agency: NIDDK NIH HHS, Id: R01 DK067166-02
  • Agency: NIDDK NIH HHS, Id: T32 DK007542
  • Agency: NIDDK NIH HHS, Id: T32 DK007542-20
  • Agency: NIDDK NIH HHS, Id: T32 DK007542-21
  • Agency: NIDDK NIH HHS, Id: T32-DK007542

Mesh Terms

  • Animals
  • Chromosomes, Artificial, Bacterial
  • Fluorescence
  • Green Fluorescent Proteins
  • Mice
  • Mice, Transgenic
  • Proteins
  • RNA, Untranslated