• Register
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.


Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.


Recruitment of chromatin-modifying enzymes by CTIP2 promotes HIV-1 transcriptional silencing.

Following entry and reverse transcription, the HIV-1 genome is integrated into the host genome. In contrast to productively infected cells, latently infected cells frequently harbor HIV-1 genomes integrated in heterochromatic structures, allowing persistence of transcriptionally silent proviruses. Microglial cells are the main HIV-1 target cells in the central nervous system and constitute an important reservoir for viral pathogenesis. In the present work, we show that, in microglial cells, the co-repressor COUP-TF interacting protein 2 (CTIP2) recruits a multienzymatic chromatin-modifying complex and establishes a heterochromatic environment at the HIV-1 promoter. We report that CTIP2 recruits histone deacetylase (HDAC)1 and HDAC2 to promote local histone H3 deacetylation at the HIV-1 promoter region. In addition, DNA-bound CTIP2 also associates with the histone methyltransferase SUV39H1, which increases local histone H3 lysine 9 methylation. This allows concomitant recruitment of HP1 proteins to the viral promoter and formation of local heterochromatin, leading to HIV-1 silencing. Altogether, our findings uncover new therapeutic opportunities for purging latent HIV-1 viruses from their cellular reservoirs.

Pubmed ID: 17245431


  • Marban C
  • Suzanne S
  • Dequiedt F
  • de Walque S
  • Redel L
  • Van Lint C
  • Aunis D
  • Rohr O


The EMBO journal

Publication Data

January 24, 2007

Associated Grants


Mesh Terms

  • Cells, Cultured
  • Chromatin
  • DNA-Binding Proteins
  • Gene Silencing
  • HIV-1
  • Histone Deacetylase 1
  • Histone Deacetylase 2
  • Histone Deacetylases
  • Histone-Lysine N-Methyltransferase
  • Histones
  • Humans
  • Methyltransferases
  • Models, Biological
  • Promoter Regions, Genetic
  • Protein Binding
  • Protein Methyltransferases
  • Repressor Proteins
  • Transcription, Genetic
  • Tumor Suppressor Proteins
  • Virus Replication