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Abnormal sperm in mice lacking the Taf7l gene.

TFIID is a general transcription factor required for transcription of most protein-coding genes by RNA polymerase II. TAF7L is an X-linked germ cell-specific paralogue of TAF7, which is a generally expressed component of TFIID. Here, we report the generation of Taf7l mutant mice by homologous recombination in embryonic stem cells by using the Cre-loxP strategy. While spermatogenesis was completed in Taf7l(-/Y) mice, the weight of Taf7l(-/Y) testis decreased and the amount of sperm in the epididymides was sharply reduced. Mutant epididymal sperm exhibited abnormal morphology, including folded tails. Sperm motility was significantly reduced, and Taf7l(-/Y) males were fertile with reduced litter size. Microarray profiling revealed that the abundance of six gene transcripts (including Fscn1) in Taf7l(-/Y) testes decreased more than twofold. In particular, FSCN1 is an F-action-bundling protein and thus may be critical for normal sperm morphology and sperm motility. Although deficiency of Taf7l may be compensated in part by Taf7, Taf7l has apparently evolved new specialized functions in the gene-selective transcription in male germ cell differentiation. Our mouse studies suggest that mutations in the human TAF7L gene might be implicated in X-linked oligozoospermia in men.

Pubmed ID: 17242199

Authors

  • Cheng Y
  • Buffone MG
  • Kouadio M
  • Goodheart M
  • Page DC
  • Gerton GL
  • Davidson I
  • Wang PJ

Journal

Molecular and cellular biology

Publication Data

April 14, 2007

Associated Grants

  • Agency: NICHD NIH HHS, Id: 1R01 HD 41552
  • Agency: FIC NIH HHS, Id: 5D43 TW 00671
  • Agency: NICHD NIH HHS, Id: HD 045866
  • Agency: NICHD NIH HHS, Id: U01 HD045866
  • Agency: NICHD NIH HHS, Id: U01 HD045866-04

Mesh Terms

  • Animals
  • Cell Differentiation
  • Cell Movement
  • Epididymis
  • Female
  • Fertility
  • Gene Expression Profiling
  • Litter Size
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mutation
  • Oligonucleotide Array Sequence Analysis
  • Spermatogenesis
  • Spermatozoa
  • Testis
  • Transcription Factor TFIID