• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

All four CatSper ion channel proteins are required for male fertility and sperm cell hyperactivated motility.

Mammalian spermatozoa become motile at ejaculation, but before they can fertilize the egg, they must acquire more thrust to penetrate the cumulus and zona pellucida. The forceful asymmetric motion of hyperactivated spermatozoa requires Ca2+ entry into the sperm tail by an alkalinization-activated voltage-sensitive Ca2+-selective current (ICatSper). Hyperactivation requires CatSper1 and CatSper2 putative ion channel genes, but the function of two other related genes (CatSper3 and CatSper4) is not known. Here we show that targeted disruption of murine CatSper3 or CatSper4 also abrogated ICatSper, sperm cell hyperactivated motility and male fertility but did not affect spermatogenesis or initial motility. Direct protein interactions among CatSpers, the sperm specificity of these proteins, and loss of ICatSper in each of the four CatSper-/- mice indicate that CatSpers are highly specialized flagellar proteins.

Pubmed ID: 17227845

Authors

  • Qi H
  • Moran MM
  • Navarro B
  • Chong JA
  • Krapivinsky G
  • Krapivinsky L
  • Kirichok Y
  • Ramsey IS
  • Quill TA
  • Clapham DE

Journal

Proceedings of the National Academy of Sciences of the United States of America

Publication Data

January 23, 2007

Associated Grants

  • Agency: NICHD NIH HHS, Id: HD045339
  • Agency: NICHD NIH HHS, Id: HD36022
  • Agency: PHS HHS, Id: U01 45857

Mesh Terms

  • Animals
  • Calcium Channels
  • Fertility
  • Male
  • Mice
  • Molecular Sequence Data
  • Protein Isoforms
  • Sperm Motility