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Postnatal deletion of Numb/Numblike reveals repair and remodeling capacity in the subventricular neurogenic niche.

Neural stem cells are retained in the postnatal subventricular zone (SVZ), a specialized neurogenic niche with unique cytoarchitecture and cell-cell contacts. Although the SVZ stem cells continuously regenerate, how they and the niche respond to local changes is unclear. Here we generated nestin-creER(tm) transgenic mice with inducible Cre recombinase in the SVZ and removed Numb/Numblike, key regulators of embryonic neurogenesis from postnatal SVZ progenitors and ependymal cells. This resulted in severe damage to brain lateral ventricle integrity and identified roles for Numb/Numblike in regulating ependymal wall integrity and SVZ neuroblast survival. Surprisingly, the ventricular damage was eventually repaired: SVZ reconstitution and ventricular wall remodeling were mediated by progenitors that escaped Numb deletion. Our results show a self-repair mechanism in the mammalian brain and may have implications for both niche plasticity in other areas of stem cell biology and the therapeutic use of neural stem cells in neurodegenerative diseases.

Pubmed ID: 17174898

Authors

  • Kuo CT
  • Mirzadeh Z
  • Soriano-Navarro M
  • Rasin M
  • Wang D
  • Shen J
  • Sestan N
  • Garcia-Verdugo J
  • Alvarez-Buylla A
  • Jan LY
  • Jan YN

Journal

Cell

Publication Data

December 15, 2006

Associated Grants

  • Agency: NINDS NIH HHS, Id: 5 R01 NS047200
  • Agency: NINDS NIH HHS, Id: NS28478
  • Agency: NICHD NIH HHS, Id: R01 HD045481
  • Agency: NIMH NIH HHS, Id: R01 MH084234
  • Agency: NINDS NIH HHS, Id: R01 NS047200
  • Agency: NINDS NIH HHS, Id: R37 NS040929

Mesh Terms

  • Animals
  • Animals, Newborn
  • Brain
  • Cell Communication
  • Ependyma
  • Female
  • Gene Deletion
  • Integrases
  • Intermediate Filament Proteins
  • Lateral Ventricles
  • Male
  • Membrane Proteins
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Nerve Tissue Proteins
  • Nestin
  • Neurons
  • Stem Cells