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Functional interaction between PML and SATB1 regulates chromatin-loop architecture and transcription of the MHC class I locus.

The function of the subnuclear structure the promyelocytic leukaemia (PML) body is unclear largely because of the functional heterogeneity of its constituents. Here, we provide the evidence for a direct link between PML, higher-order chromatin organization and gene regulation. We show that PML physically and functionally interacts with the matrix attachment region (MAR)-binding protein, special AT-rich sequence binding protein 1 (SATB1) to organize the major histocompatibility complex (MHC) class I locus into distinct higher-order chromatin-loop structures. Interferon gamma (IFNgamma) treatment and silencing of either SATB1 or PML dynamically alter chromatin architecture, thus affecting the expression profile of a subset of MHC class I genes. Our studies identify PML and SATB1 as a regulatory complex that governs transcription by orchestrating dynamic chromatin-loop architecture.

Pubmed ID: 17173041

Authors

  • Kumar PP
  • Bischof O
  • Purbey PK
  • Notani D
  • Urlaub H
  • Dejean A
  • Galande S

Journal

Nature cell biology

Publication Data

January 2, 2007

Associated Grants

  • Agency: Wellcome Trust, Id:

Mesh Terms

  • Cell Line
  • Cell Nucleus
  • Chromatin
  • Gene Expression Regulation
  • Genes, MHC Class I
  • Humans
  • Interferon-gamma
  • Leukemia, Promyelocytic, Acute
  • Matrix Attachment Region Binding Proteins
  • Matrix Attachment Regions
  • Models, Molecular
  • Protein Isoforms
  • RNA Interference
  • Transcription, Genetic
  • Transfection