Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Ndfip1 protein promotes the function of itch ubiquitin ligase to prevent T cell activation and T helper 2 cell-mediated inflammation.

Immunity | Dec 18, 2006

http://www.ncbi.nlm.nih.gov/pubmed/17137798

Nedd4 family interacting protein-1 (Ndfip1) is a protein whose only known function is that it binds Nedd4, a HECT-type E3 ubiquitin ligase. Here we show that mice lacking Ndfip1 developed severe inflammation of the skin and lung and died prematurely. This condition was due to a defect in Ndfip1(-/-) T cells. Ndfip1(-/-) T cells were activated, and they proliferated and adopted a T helper 2 (Th2) phenotype more readily than did their Ndfip1(+/+) counterparts. This phenotype resembled that of Itchy mutant mice, suggesting that Ndfip1 might affect the function of Itch, an E3 ubiquitin ligase. We show that T cell activation promoted both Ndfip1 expression and its association with Itch. In the absence of Ndfip1, JunB half-life was prolonged after T cell activation. Thus, in the absence of Ndfip1, Itch is inactive and JunB accumulates. As a result, T cells produce Th2 cytokines and promote Th2-mediated inflammatory disease.

Pubmed ID: 17137798 RIS Download

Mesh terms: Animals | Blotting, Western | Carrier Proteins | Cytokines | Flow Cytometry | Inflammation | Lymphocyte Activation | Membrane Proteins | Mice | Proto-Oncogene Proteins c-jun | Reverse Transcriptase Polymerase Chain Reaction | T-Lymphocytes | Th2 Cells | Ubiquitin-Protein Ligases

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NIAID NIH HHS, Id: AI-22295
  • Agency: NIAID NIH HHS, Id: AI-52225
  • Agency: NIDDK NIH HHS, Id: P50 DK052617-100007
  • Agency: NIDDK NIH HHS, Id: P50 DK52617
  • Agency: NHLBI NIH HHS, Id: R01 HL105834

Mouse Genome Informatics (Data, Gene Annotation)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.