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BCR sequences essential for transformation by the BCR-ABL oncogene bind to the ABL SH2 regulatory domain in a non-phosphotyrosine-dependent manner.

Cell | Jul 12, 1991

http://www.ncbi.nlm.nih.gov/pubmed/1712671

BCR-ABL is a chimeric oncogene implicated in the pathogenesis of Philadelphia chromosome-positive human leukemias. BCR first exon sequences specifically activate the tyrosine kinase and transforming potential of BCR-ABL. We have tested the hypothesis that activation of BCR-ABL may involve direct interaction between BCR sequences and the tyrosine kinase regulatory domains of ABL. Full-length c-BCR as well as BCR sequences retained in BCR-ABL bind specifically to the SH2 domain of ABL. The binding domain has been localized within the first exon of BCR and consists of at least two SH2-binding sites. This domain is essential for BCR-ABL-mediated transformation. Phosphoserine/phosphothreonine but not phosphotyrosine residues on BCR are required for interaction with the ABL SH2 domain. These findings extend the range of potential SH2-protein interactions in growth control pathways and suggest a function for SH2 domains in the activation of the BCR-ABL oncogene as well as a role for BCR in cellular signaling pathways.

Pubmed ID: 1712671 RIS Download

Mesh terms: Amino Acid Sequence | Animals | Binding Sites | Cell Line | Cell Transformation, Neoplastic | Exons | Fusion Proteins, bcr-abl | Genes, abl | Genetic Variation | Humans | Insects | Molecular Sequence Data | Oncogene Proteins | Oncogenes | Phosphotyrosine | Plasmids | Protein Biosynthesis | Protein-Tyrosine Kinases | Proto-Oncogene Proteins | Proto-Oncogene Proteins c-bcr | Regulatory Sequences, Nucleic Acid | Transcription, Genetic | Transfection | Tyrosine