Literature search services are currently unavailable. During our hosting provider's UPS upgrade we experienced a hardware failure and are currently working to resolve the issue.

Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Direct interaction between DNMT1 and G9a coordinates DNA and histone methylation during replication.

Chromatin methylation is necessary for stable repression of gene expression during mammalian development. During cell division, DNMT1 maintains the DNA methylation pattern of the newly synthesized daughter strand, while G9a methylates H3K9. Here, DNMT1 is shown to directly bind G9a both in vivo and in vitro and to colocalize in the nucleus during DNA replication. The complex of DNMT1 and G9a colocalizes with dimethylated H3K9 (H3K9me2) at replication foci. Similarly, another H3K9 histone methyltransferase, SUV39H1, colocalizes with DNMT1 on heterochromatic regions of the nucleoli exclusively before cell division. Both DNMT1 and G9a are loaded onto the chromatin simultaneously in a ternary complex with loading factor PCNA during chromatin replication. Small interfering RNA (siRNA) knockdown of DNMT1 impairs DNA methylation, G9a loading, and H3K9 methylation on chromatin and rDNA repeats, confirming DNMT1 as the primary loading factor. Additionally, the complex of DNMT1 and G9a led to enhanced DNA and histone methylation of in vitro assembled chromatin substrates. Thus, direct cooperation between DNMT1 and G9a provides a mechanism of coordinated DNA and H3K9 methylation during cell division.

Pubmed ID: 17085482


  • Estève PO
  • Chin HG
  • Smallwood A
  • Feehery GR
  • Gangisetty O
  • Karpf AR
  • Carey MF
  • Pradhan S


Genes & development

Publication Data

November 15, 2006

Associated Grants

  • Agency: NCI NIH HHS, Id: R01 CA116674
  • Agency: NCI NIH HHS, Id: R01 CA116674-02
  • Agency: NCI NIH HHS, Id: R01 CA11674

Mesh Terms

  • Animals
  • COS Cells
  • Cercopithecus aethiops
  • DNA (Cytosine-5-)-Methyltransferase
  • DNA Methylation
  • DNA Replication
  • DNA, Ribosomal Spacer
  • HCT116 Cells
  • HeLa Cells
  • Heterochromatin
  • Histone-Lysine N-Methyltransferase
  • Histones
  • Humans
  • Jurkat Cells
  • Methyltransferases
  • Models, Genetic
  • Protein Binding
  • Protein Methyltransferases
  • Protein Transport
  • Repressor Proteins