Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Opposing roles for Set2 and yFACT in regulating TBP binding at promoters.

The EMBO journal | Oct 4, 2006

Previous work links histone methylation by Set2 with transcriptional elongation. yFACT (Spt16-Pob3 and Nhp6) reorganizes nucleosomes and functions in both transcriptional initiation and elongation. We show that growth defects caused by spt16 or pob3 mutations can be suppressed by deleting SET2, suggesting that Set2 and yFACT have opposing roles. Set2 methylates K36 of histone H3, and K36 substitutions also suppress yFACT mutations. In contrast, set1 enhances yFACT mutations. Methylation at H3 K4 by Set1 is required for set2 to suppress yFACT defects. We did not detect an elongation defect at an 8 kb ORF in yFACT mutants. Instead, pob3 mutants displayed reduced binding of both pol II and TBP to the GAL1 promoter. Importantly, both GAL1 transcription and promoter binding of pol II and TBP are significantly restored in the pob3 set2 double mutant. Defects caused by an spt16 mutation are enhanced by either TBP or TFIIA mutants. These synthetic defects are suppressed by set2, demonstrating that yFACT and Set2 oppose one another during transcriptional initiation at a step involving DNA binding by TBP and TFIIA.

Pubmed ID: 16977311 RIS Download

Mesh terms: Amino Acid Substitution | Carrier Proteins | Cell Cycle Proteins | DNA-Binding Proteins | Epistasis, Genetic | Gene Expression Regulation, Fungal | Histone-Lysine N-Methyltransferase | Histones | Methylation | Methyltransferases | Mutant Proteins | Mutation | Phenotype | Promoter Regions, Genetic | Protein Binding | RNA Polymerase II | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | TATA-Box Binding Protein | Temperature | Transcription Factor TFIIA | Transcription Factors | Transcriptional Elongation Factors

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

Associated grants


Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.