Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Graded regulation of the Kv2.1 potassium channel by variable phosphorylation.

Science (New York, N.Y.) | Aug 18, 2006

Dynamic modulation of ion channels by phosphorylation underlies neuronal plasticity. The Kv2.1 potassium channel is highly phosphorylated in resting mammalian neurons. Activity-dependent Kv2.1 dephosphorylation by calcineurin induces graded hyperpolarizing shifts in voltage-dependent activation, causing suppression of neuronal excitability. Mass spectrometry-SILAC (stable isotope labeling with amino acids in cell culture) identified 16 Kv2.1 phosphorylation sites, of which 7 were dephosphorylated by calcineurin. Mutation of individual calcineurin-regulated sites to alanine produced incremental shifts mimicking dephosphorylation, whereas mutation to aspartate yielded equivalent resistance to calcineurin. Mutations at multiple sites were additive, showing that variable phosphorylation of Kv2.1 at a large number of sites allows graded activity-dependent regulation of channel gating and neuronal firing properties.

Pubmed ID: 16917065 RIS Download

Mesh terms: Alanine | Alkaline Phosphatase | Animals | Aspartic Acid | Brain | Calcineurin | Calcium | Cell Line | Chromatography, Liquid | Humans | Ion Channel Gating | Ionomycin | Mass Spectrometry | Mutation | Neurons | Patch-Clamp Techniques | Phosphorylation | Phosphoserine | Phosphothreonine | Point Mutation | Rats | Recombinant Proteins | Serine | Shab Potassium Channels | Transfection

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NINDS NIH HHS, Id: R01 NS042225
  • Agency: NINDS NIH HHS, Id: R01 NS042225-06
  • Agency: NINDS NIH HHS, Id: NS42225

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

This is a list of tools and resources that we have found mentioned in this publication.


Anti-Kv2.1-pS453 phosphospecific rabbit polyclonal antibody

Rat Kv2.1 synthetic phosphopeptide amino acids 447-467, KDAFAR[pS]IEMMDIVVEKNGES, accession NP_037318, kdafarsiemmdivveknges

antibody

View all literature mentions

Anti-Kv2.1-pS563 phosphospecific rabbit polyclonal antibody

Rat Kv2.1 synthetic peptide amino acids 554-574, accession NP_037318

antibody

View all literature mentions

Anti-Kv2.1-pS603 L61/14 phosphospecific mouse monoclonal antibody L61/14

Rat Kv2.1 synthetic phosphopeptide amino acids 595–616, CPEATRFSH[pS]PLASLSSKAGSS, accession NP_037318

antibody

View all literature mentions

Anti-Kv2.1-pS715 phosphospecific rabbit polyclonal antibody

Rat Kv2.1 synthetic peptide amino acids 710-728, CDKPVLpSPESSIYTTASART, accession NP_037318

antibody

View all literature mentions