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The inner nuclear membrane protein emerin regulates beta-catenin activity by restricting its accumulation in the nucleus.

Emerin is a type II inner nuclear membrane (INM) protein of unknown function. Emerin function is likely to be important because, when it is mutated, emerin promotes both skeletal muscle and heart defects. Here we show that one function of Emerin is to regulate the flux of beta-catenin, an important transcription coactivator, into the nucleus. Emerin interacts with beta-catenin through a conserved adenomatous polyposis coli (APC)-like domain. When GFP-emerin is expressed in HEK293 cells, beta-catenin is restricted to the cytoplasm and beta-catenin activity is inhibited. In contrast, expression of an emerin mutant, lacking its APC-like domain (GFP-emerinDelta), dominantly stimulates beta-catenin activity and increases nuclear accumulation of beta-catenin. Human fibroblasts that are null for emerin have an autostimulatory growth phenotype. This unusual growth phenotype arises through enhanced nuclear accumulation and activity of beta-catenin and can be replicated in wild-type fibroblasts by transfection with constitutively active beta-catenin. Our results support recent findings that suggest that INM proteins can influence signalling pathways by restricting access of transcription coactivators to the nucleus.

Pubmed ID: 16858403

Authors

  • Markiewicz E
  • Tilgner K
  • Barker N
  • van de Wetering M
  • Clevers H
  • Dorobek M
  • Hausmanowa-Petrusewicz I
  • Ramaekers FC
  • Broers JL
  • Blankesteijn WM
  • Salpingidou G
  • Wilson RG
  • Ellis JA
  • Hutchison CJ

Journal

The EMBO journal

Publication Data

July 26, 2006

Associated Grants

  • Agency: Medical Research Council, Id: G0500501
  • Agency: Wellcome Trust, Id:

Mesh Terms

  • Cell Line
  • Cells, Cultured
  • Humans
  • Membrane Proteins
  • Muscular Dystrophy, Emery-Dreifuss
  • Nuclear Envelope
  • Nuclear Proteins
  • Signal Transduction
  • Thymopoietins
  • Trans-Activators
  • beta Catenin