• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Genetic regulation of unsaturated fatty acid composition in C. elegans.

Delta-9 desaturases, also known as stearoyl-CoA desaturases, are lipogenic enzymes responsible for the generation of vital components of membranes and energy storage molecules. We have identified a novel nuclear hormone receptor, NHR-80, that regulates delta-9 desaturase gene expression in Caenorhabditis elegans. Here we describe fatty acid compositions, lifespans, and gene expression studies of strains carrying mutations in nhr-80 and in the three genes encoding delta-9 desaturases, fat-5, fat-6, and fat-7. The delta-9 desaturase single mutants display only subtle changes in fatty acid composition and no other visible phenotypes, yet the fat-5;fat-6;fat-7 triple mutant is lethal, revealing that endogenous production of monounsaturated fatty acids is essential for survival. In the absence of FAT-6 or FAT-7, the expression of the remaining desaturases increases, and this ability to compensate depends on NHR-80. We conclude that, like mammals, C. elegans requires adequate synthesis of unsaturated fatty acids and maintains complex regulation of the delta-9 desaturases to achieve optimal fatty acid composition.

Pubmed ID: 16839188

Authors

  • Brock TJ
  • Browse J
  • Watts JL

Journal

PLoS genetics

Publication Data

July 14, 2006

Associated Grants

  • Agency: NIDDK NIH HHS, Id: R01 DK074114
  • Agency: NIDDK NIH HHS, Id: R01 DK074114-01
  • Agency: NIDDK NIH HHS, Id: R01-DK074114

Mesh Terms

  • Animals
  • Caenorhabditis elegans
  • Fatty Acids, Unsaturated
  • Gene Expression Regulation
  • Green Fluorescent Proteins
  • Mutation
  • Stearoyl-CoA Desaturase
  • Substrate Specificity