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A DNA insulator prevents repression of a targeted X-linked transgene but not its random or imprinted X inactivation.

Some genes on the inactive X chromosome escape silencing. One possible escape mechanism is that heterochromatization during X inactivation can be blocked by boundary elements. DNA insulators are candidates for blocking because they shield genes from influences of their chromosomal environment. To test whether DNA insulators can act as boundaries on the X chromosome, we inserted into the mouse X-linked Hprt locus a GFP transgene flanked with zero, one, or two copies of a prototypic vertebrate insulator from the chicken beta-globin locus, chicken hypersensitive site 4, which contains CCCTC binding factor binding sites. On the active X chromosome the insulators blocked repression of the transgene, which commences during early development and persists in adults, in a copy number-dependent manner. CpG methylation of the transgene correlated inversely with expression, but the insulators on the active X chromosome were not methylated. On the inactive X chromosome, insulators did not block random or imprinted X inactivation of the transgene, and both the insulator and transgene were almost completely methylated. Thus, the chicken hypersensitive site 4 DNA insulator is sufficient to protect an X-linked gene from repression during development but not from X inactivation.

Pubmed ID: 16777957

Authors

  • Ciavatta D
  • Kalantry S
  • Magnuson T
  • Smithies O

Journal

Proceedings of the National Academy of Sciences of the United States of America

Publication Data

June 27, 2006

Associated Grants

  • Agency: NHLBI NIH HHS, Id: HL 37001
  • Agency: NHLBI NIH HHS, Id: R01 HL037001

Mesh Terms

  • Animals
  • Chickens
  • Female
  • Gene Expression
  • Genes, Reporter
  • Genes, X-Linked
  • Genomic Imprinting
  • Green Fluorescent Proteins
  • Insulator Elements
  • Male
  • Mice
  • Mice, Transgenic
  • Transgenes
  • X Chromosome Inactivation