• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Neural crest cells retain multipotential characteristics in the developing valves and label the cardiac conduction system.

Multipotent neural crest cells (NCCs) are a major extracardiac component of cardiovascular development. Although recognized as contributing cells to the arterial valves at early developmental stages, NCC persistence in the valves at later times or in the adult heart is controversial. We analyzed NCC persistence and contributions to both semilunar and atrioventricular (AV) valves in the mature heart. Two NCC-specific promoters driving Cre recombinase, Wnt1-Cre and P0-Cre, were mated with floxed reporter mice, R26R or CAG-CAT-EGFP, to map NCC fate. Hearts were analyzed before aorticopulmonary (AP) septation through adult stages. As previously demonstrated, strong NCC labeling was detected in ventral and dorsal outflow cushions before AP septation. In contrast to previous reports, we found that substantial numbers of labeled cells persisted in the semilunar valves in late fetal, neonatal, and adult hearts. Furthermore, NCCs were also found in the AV valves, almost exclusively in the septal leaflets. NCCs in the AV valves expressed melanocytic and neurogenic markers. However, cells labeled in the proximal cardiac conduction system exhibited neurogenic and gliagenic markers, whereas some NCCs expressed no differentiation specific markers. These results suggest that cardiac NCCs contribute to the mature valves and the cardiac conduction system and retain multipotent characteristics late in development.

Pubmed ID: 16709902

Authors

  • Nakamura T
  • Colbert MC
  • Robbins J

Journal

Circulation research

Publication Data

June 23, 2006

Associated Grants

  • Agency: NHLBI NIH HHS, Id: HL074728
  • Agency: NHLBI NIH HHS, Id: HL52318
  • Agency: NHLBI NIH HHS, Id: HL56370
  • Agency: NHLBI NIH HHS, Id: HL61638
  • Agency: NHLBI NIH HHS, Id: HL69779

Mesh Terms

  • Aging
  • Animals
  • Animals, Newborn
  • Aorta, Thoracic
  • Biological Markers
  • Cell Differentiation
  • Cell Lineage
  • Embryonic Development
  • Green Fluorescent Proteins
  • Heart Conduction System
  • Heart Valves
  • Mice
  • Mice, Transgenic
  • Nervous System
  • Neural Crest