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Genetic analysis of synaptotagmin 2 in spontaneous and Ca2+-triggered neurotransmitter release.

The EMBO journal | May 17, 2006

http://www.ncbi.nlm.nih.gov/pubmed/16642042

Synaptotagmin 2 resembles synaptotagmin 1, the Ca2+ sensor for fast neurotransmitter release in forebrain synapses, but little is known about synaptotagmin 2 function. Here, we describe a severely ataxic mouse strain that harbors a single, destabilizing amino-acid substitution (I377N) in synaptotagmin 2. In Calyx of Held synapses, this mutation causes a delay and a decrease in Ca2+-induced but not in hypertonic sucrose-induced release, suggesting that synaptotagmin 2 mediates Ca2+ triggering of evoked release in brainstem synapses. Unexpectedly, we additionally observed in synaptotagmin 2 mutant synapses a dramatic increase in spontaneous release. Synaptotagmin 1-deficient excitatory and inhibitory cortical synapses also displayed a large increase in spontaneous release, demonstrating that this effect was shared among synaptotagmins 1 and 2. Our data suggest that synaptotagmin 1 and 2 perform equivalent functions in the Ca2+ triggering of action potential-induced release and in the restriction of spontaneous release, consistent with a general role of synaptotagmins in controlling 'release slots' for synaptic vesicles at the active zone.

Pubmed ID: 16642042 RIS Download

Mesh terms: Action Potentials | Amino Acid Sequence | Animals | Ataxia | Brain | Calcium | In Vitro Techniques | Mice | Molecular Sequence Data | Neurons | Neurotransmitter Agents | Patch-Clamp Techniques | Point Mutation | Protein Denaturation | Protein Structure, Tertiary | Sequence Alignment | Synapses | Synaptic Transmission | Synaptotagmin I | Synaptotagmin II

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Mouse Genome Informatics (Data, Gene Annotation)

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