Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Synaptopodin orchestrates actin organization and cell motility via regulation of RhoA signalling.

Nature cell biology | May 12, 2006

http://www.ncbi.nlm.nih.gov/pubmed/16622418

The Rho family of small GTPases (RhoA, Rac1 and Cdc42) controls signal-transduction pathways that influence many aspects of cell behaviour, including cytoskeletal dynamics. At the leading edge, Rac1 and Cdc42 promote cell motility through the formation of lamellipodia and filopodia, respectively. On the contrary, RhoA promotes the formation of contractile actin-myosin-containing stress fibres in the cell body and at the rear. Here, we identify synaptopodin, an actin-associated protein, as a novel regulator of RhoA signalling and cell migration in kidney podocytes. We show that synaptopodin induces stress fibres by competitive blocking of Smurf1-mediated ubiquitination of RhoA, thereby preventing the targeting of RhoA for proteasomal degradation. Gene silencing of synaptopodin in kidney podocytes causes the loss of stress fibres and the formation of aberrant non-polarized filopodia and impairment of cell migration. Together, these data show that synaptopodin is essential for the integrity of the podocyte actin cytoskeleton and for the regulation of podocyte cell migration.

Pubmed ID: 16622418 RIS Download

Mesh terms: Actins | Cell Movement | Cells, Cultured | Cytoskeletal Proteins | Humans | Microfilament Proteins | Podocytes | Protein Processing, Post-Translational | Protein Transport | RNA, Messenger | Recombinant Fusion Proteins | Signal Transduction | Stress Fibers | Ubiquitin-Protein Ligases | Up-Regulation | rhoA GTP-Binding Protein

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.