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Expression cloning and characterization of the TGF-beta type III receptor.

The rat TGF-beta type III receptor cDNA has been cloned by overexpression in COS cells. The encoded receptor is an 853 amino acid protein with a large N-terminal extracellular domain containing at least one site for glycosaminoglycan addition, a single hydrophobic transmembrane domain, and a 41 amino acid cytoplasmic tail with no obvious signaling motif. Introduction of the cDNA into COS cells and L6 myoblasts induces expression of a heterogenously glycosylated 280-330 kd protein characteristic of the type III receptor that binds TGF-beta 1 specifically. In L6 myoblasts lacking the endogenous type III receptor, expression of the recombinant receptor leads to an increase in the amount of ligand bound and cross-linked to surface type II TGF-beta receptors. This indicates that the type III receptor may regulate the ligand-binding ability or surface expression of the type II receptor.

Pubmed ID: 1657407


  • Wang XF
  • Lin HY
  • Ng-Eaton E
  • Downward J
  • Lodish HF
  • Weinberg RA



Publication Data

November 15, 1991

Associated Grants

  • Agency: NHLBI NIH HHS, Id: HL-41484
  • Agency: NCI NIH HHS, Id: R35-CA39826
  • Agency: NIGMS NIH HHS, Id: T 32 GM07287-16

Mesh Terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA
  • Gene Expression
  • Membrane Glycoproteins
  • Membrane Proteins
  • Molecular Sequence Data
  • Proteoglycans
  • RNA, Messenger
  • Rats
  • Receptors, Cell Surface
  • Receptors, Transforming Growth Factor beta
  • Transfection
  • Transforming Growth Factor beta