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Expression cloning and characterization of the TGF-beta type III receptor.

Cell | Nov 15, 1991

http://www.ncbi.nlm.nih.gov/pubmed/1657407

The rat TGF-beta type III receptor cDNA has been cloned by overexpression in COS cells. The encoded receptor is an 853 amino acid protein with a large N-terminal extracellular domain containing at least one site for glycosaminoglycan addition, a single hydrophobic transmembrane domain, and a 41 amino acid cytoplasmic tail with no obvious signaling motif. Introduction of the cDNA into COS cells and L6 myoblasts induces expression of a heterogenously glycosylated 280-330 kd protein characteristic of the type III receptor that binds TGF-beta 1 specifically. In L6 myoblasts lacking the endogenous type III receptor, expression of the recombinant receptor leads to an increase in the amount of ligand bound and cross-linked to surface type II TGF-beta receptors. This indicates that the type III receptor may regulate the ligand-binding ability or surface expression of the type II receptor.

Pubmed ID: 1657407 RIS Download

Mesh terms: Amino Acid Sequence | Animals | Base Sequence | Cloning, Molecular | DNA | Gene Expression | Membrane Glycoproteins | Membrane Proteins | Molecular Sequence Data | Proteoglycans | RNA, Messenger | Rats | Receptors, Cell Surface | Receptors, Transforming Growth Factor beta | Transfection | Transforming Growth Factor beta

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