Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

mTOR-dependent stimulation of the association of eIF4G and eIF3 by insulin.

The EMBO journal | Apr 19, 2006

Insulin stimulates protein synthesis by increasing translation initiation. This response is mediated by mTOR and is believed to result from 4EBP1 phosphorylation, which allows eIF4E to bind eIF4G. Here, we present evidence that mTOR interacts directly with eIF3 and that mTOR controls the association of eIF3 and eIF4G. Activating mTOR signaling with insulin increased by as much as five-fold the amount of eIF4G bound to eIF3. This novel effect was blocked by rapamycin and other inhibitors of mTOR, and it required neither eIF4E binding to eIF4G nor eIF3 binding to the 40S ribosomal subunit. The increase in eIF4G associated with eIF3 occurred rapidly and at physiological concentrations of insulin. Moreover, the magnitude of the response was similar to the increase in eIF4E binding to eIF4G produced by insulin. Thus, increasing eIF4G association with eIF3 represents a potentially important mechanism by which insulin, as well as amino acids and growth factors that activate mTOR, stimulate translation.

Pubmed ID: 16541103 RIS Download

Mesh terms: Adipocytes | Amino Acid Sequence | Animals | Cell Line | Eukaryotic Initiation Factor-3 | Eukaryotic Initiation Factor-4G | Humans | Insulin | Mice | Molecular Sequence Data | Mutation | Phosphorylation | Protein Binding | Protein Biosynthesis | Protein Kinase Inhibitors | Protein Kinases | Protein Subunits | Ribosomal Proteins | Signal Transduction | Sirolimus | TOR Serine-Threonine Kinases | Two-Hybrid System Techniques

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NIDDK NIH HHS, Id: DK28312
  • Agency: NIDDK NIH HHS, Id: DK52753
  • Agency: NIGMS NIH HHS, Id: GM20818
  • Agency: NIGMS NIH HHS, Id: GM37537

BioGRID (Data, Interactions)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.