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Coactivator-associated arginine methyltransferase-1 enhances nuclear factor-kappaB-mediated gene transcription through methylation of histone H3 at arginine 17.

Coactivator-associated arginine methyltransferase-1 (CARM1) is known to enhance transcriptional activation by nuclear receptors through interactions with the coactivators p160 and cAMP response element binding protein-binding protein (CBP) and methylation of histone H3 at arginine 17 (H3-R17). Here, we show that CARM1 can act as a coactivator for the transcription factor nuclear factor-kappaB (NF-kappaB) and enhance NF-kappaB activity in a CBP (p300)-dependent manner. This enhancement in 293T cells was abolished by cotransfection with a specific short hairpin RNA targeted to knockdown CARM1. Chromatin immunoprecipitation demonstrated CARM1 recruitment in vivo to the promoters of NF-kappaB p65-regulated genes along with CBP and steroid receptor coactivator-1. This was accompanied by an increase in histone H3-R17 methylation as well as H3-K9 and H3-K14 acetylation, and a decrease in H3-citrulline. Immunoprecipitation with anti-p65 antibody revealed that CARM1 physically interacts with NF-kappaB p65. Furthermore, we demonstrated the physiological significance by observing that similar events occurred when THP-1 monocytic cells were stimulated with TNF-alpha or with S100b, a ligand for the receptor of advanced glycation end products, both of which are associated with diabetic complications and also known inducers of NF-kappaB and inflammatory genes in monocytes. These results demonstrate that CARM1 participates in NF-kappaB-mediated transcription through H3-R17 methylation and support a nonnuclear receptor-associated function for CARM1. They also demonstrate for the first time that CARM1 occupancy, histone H3-R17 methylation, and citrullination are regulated at the promoters of inflammatory genes in monocytes, thereby suggesting a novel role for histone arginine modifications in inflammatory diseases.

Pubmed ID: 16497732


  • Miao F
  • Li S
  • Chavez V
  • Lanting L
  • Natarajan R


Molecular endocrinology (Baltimore, Md.)

Publication Data

July 27, 2006

Associated Grants

  • Agency: NHLBI NIH HHS, Id: P01 HL55798
  • Agency: NIDDK NIH HHS, Id: R01 DK065073
  • Agency: NIDDK NIH HHS, Id: R01 DK65073
  • Agency: NHLBI NIH HHS, Id: R01 HL087864

Mesh Terms

  • Arginine
  • CREB-Binding Protein
  • Cells, Cultured
  • Down-Regulation
  • Gene Expression Regulation
  • Histone Acetyltransferases
  • Histones
  • Humans
  • Methylation
  • Nuclear Receptor Coactivator 1
  • Protein Binding
  • Protein-Arginine N-Methyltransferases
  • RNA Interference
  • RNA, Small Interfering
  • Transcription Factor RelA
  • Transcription Factors
  • Up-Regulation