Our hosting provider will be performing UPS maintenance on Tuesday, Oct 25, 2016 between 8 AM and 5 PM PDT. SciCrunch searching services will be down during this time.

Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Complex genetic interactions in a quantitative trait locus.


Whether in natural populations or between two unrelated members of a species, most phenotypic variation is quantitative. To analyze such quantitative traits, one must first map the underlying quantitative trait loci. Next, and far more difficult, one must identify the quantitative trait genes (QTGs), characterize QTG interactions, and identify the phenotypically relevant polymorphisms to determine how QTGs contribute to phenotype. In this work, we analyzed three Saccharomyces cerevisiae high-temperature growth (Htg) QTGs (MKT1, END3, and RHO2). We observed a high level of genetic interactions among QTGs and strain background. Interestingly, while the MKT1 and END3 coding polymorphisms contribute to phenotype, it is the RHO2 3'UTR polymorphisms that are phenotypically relevant. Reciprocal hemizygosity analysis of the Htg QTGs in hybrids between S288c and ten unrelated S. cerevisiae strains reveals that the contributions of the Htg QTGs are not conserved in nine other hybrids, which has implications for QTG identification by marker-trait association. Our findings demonstrate the variety and complexity of QTG contributions to phenotype, the impact of genetic background, and the value of quantitative genetic studies in S. cerevisiae.

Pubmed ID: 16462944


  • Sinha H
  • Nicholson BP
  • Steinmetz LM
  • McCusker JH


PLoS genetics

Publication Data

February 5, 2006

Associated Grants

  • Agency: NIGMS NIH HHS, Id: R01 GM070541
  • Agency: NIGMS NIH HHS, Id: R01 GM58476

Mesh Terms

  • 3' Untranslated Regions
  • Chromosome Mapping
  • Chromosomes, Fungal
  • Gene Deletion
  • Genes, Fungal
  • Genetic Linkage
  • Models, Genetic
  • Phenotype
  • Plasmids
  • Polymorphism, Genetic
  • Quantitative Trait Loci
  • Saccharomyces cerevisiae
  • Temperature