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The disease progression of Mecp2 mutant mice is affected by the level of BDNF expression.

Neuron | Feb 2, 2006

http://www.ncbi.nlm.nih.gov/pubmed/16446138

Mutations in the MECP2 gene cause Rett syndrome (RTT). Bdnf is a MeCP2 target gene; however, its role in RTT pathogenesis is unknown. We examined Bdnf conditional mutant mice for RTT-relevant pathologies and observed that loss of BDNF caused smaller brain size, smaller CA2 neurons, smaller glomerulus size, and a characteristic hindlimb-clasping phenotype. BDNF protein level was reduced in Mecp2 mutant mice, and deletion of Bdnf in Mecp2 mutants caused an earlier onset of RTT-like symptoms. To assess whether this interaction was functional and potentially therapeutically relevant, we increased BDNF expression in the Mecp2 mutant brain with a conditional Bdnf transgene. BDNF overexpression extended the lifespan, rescued a locomotor defect, and reversed an electrophysiological deficit observed in Mecp2 mutants. Our results provide in vivo evidence for a functional interaction between Mecp2 and Bdnf and demonstrate the physiological significance of altered BDNF expression/signaling in RTT disease progression.

Pubmed ID: 16446138 RIS Download

Mesh terms: Action Potentials | Animals | Animals, Newborn | Behavior, Animal | Brain | Brain-Derived Neurotrophic Factor | Disease Models, Animal | Disease Progression | Electric Stimulation | Enzyme-Linked Immunosorbent Assay | Female | Gene Expression Regulation | Humans | Immunohistochemistry | In Vitro Techniques | Male | Methyl-CpG-Binding Protein 2 | Mice | Mice, Knockout | Motor Activity | Mutation | Neurons | Organ Size | Patch-Clamp Techniques | RNA, Messenger | Rett Syndrome | Reverse Transcriptase Polymerase Chain Reaction

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Associated grants

  • Agency: NCI NIH HHS, Id: R01 CA087869

Mouse Genome Informatics (Data, Gene Annotation)

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