Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

The Vg1-related protein Gdf3 acts in a Nodal signaling pathway in the pre-gastrulation mouse embryo.

http://www.ncbi.nlm.nih.gov/pubmed/16368929

The formation of the anterior visceral endoderm (AVE) in the pre-gastrulation mouse embryo represents a crucial event in patterning of the anterior-posterior axis. Here, we show that the transforming growth factor beta (Tgfbeta) family member Gdf3 (growth-differentiation factor 3), a close relative of Xenopus Vg1, resembles the Tgfbeta ligand Nodal in both its signaling activity and its role in AVE formation in vivo. Thus, in cell culture, Gdf3 signaling requires the EGF-CFC co-receptor Cripto and can be inhibited by Lefty antagonists. In Xenopus embryos, Gdf3 misexpression results in secondary axis formation, and induces morphogenetic elongation and mesendoderm formation in animal caps. In mouse embryos, Gdf3 is expressed in the inner cell mass and epiblast, and null mutants frequently exhibit abnormal formation or positioning of the AVE. This phenotype correlates with defects in mesoderm and definitive endoderm formation, as well as abnormal Nodal expression levels. Our findings indicate that Gdf3 acts in a Nodal-like signaling pathway in pre-gastrulation development, and provide evidence for the functional conservation of Vg1 activity in mice.

Pubmed ID: 16368929 RIS Download

Mesh terms: Animals | Base Sequence | Blastocyst | Body Patterning | DNA, Complementary | Endoderm | Epidermal Growth Factor | Gene Expression Regulation, Developmental | Growth Differentiation Factor 3 | Humans | In Vitro Techniques | Membrane Glycoproteins | Mice | Mice, Knockout | Models, Biological | Neoplasm Proteins | Nodal Protein | Phenotype | Recombinant Proteins | Signal Transduction | Transforming Growth Factor beta | Xenopus laevis

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NICHD NIH HHS, Id: HD01156
  • Agency: NICHD NIH HHS, Id: HD27823
  • Agency: NICHD NIH HHS, Id: HD32067
  • Agency: NICHD NIH HHS, Id: HD41648
  • Agency: NICHD NIH HHS, Id: HD42837
  • Agency: NHLBI NIH HHS, Id: HL67355

Mouse Genome Informatics (Data, Gene Annotation)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.