• Register
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.


Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.


Spatial restriction of PDK1 activation cascades by anchoring to mAKAPalpha.

The muscle A-kinase anchoring protein (mAKAP) tethers cAMP-dependent enzymes to perinuclear membranes of cardiomyocytes. We now demonstrate that two alternatively spliced forms of mAKAP are expressed: mAKAPalpha and mAKAPbeta. The longer form, mAKAPalpha, is preferentially expressed in the brain. mAKAPbeta is a shorter form of the anchoring protein that lacks the first 244 amino acids and is preferentially expressed in the heart. The unique amino terminus of mAKAPalpha can spatially restrict the activity of 3-phosphoinositide-dependent kinase-1 (PDK1). Biochemical and genetic analyses demonstrate that simultaneous recruitment of PDK1 and ERK onto mAKAPalpha facilitates activation and release of the downstream target p90RSK. The assembly of tissue-specific signaling complexes provides an efficient mechanism to integrate and relay lipid-mediated and mitogenic activated signals to the nucleus.

Pubmed ID: 16337591


  • Michel JJ
  • Townley IK
  • Dodge-Kafka KL
  • Zhang F
  • Kapiloff MS
  • Scott JD


Molecular cell

Publication Data

December 9, 2005

Associated Grants

  • Agency: NIDDK NIH HHS, Id: DK54441
  • Agency: NHLBI NIH HHS, Id: F32HL68480
  • Agency: NHLBI NIH HHS, Id: HL075398
  • Agency: NHLBI NIH HHS, Id: R01 HL075398
  • Agency: NHLBI NIH HHS, Id: R01 HL075398-02

Mesh Terms

  • 3-Phosphoinositide-Dependent Protein Kinases
  • A Kinase Anchor Proteins
  • Adaptor Proteins, Signal Transducing
  • Alternative Splicing
  • Amino Acid Sequence
  • Animals
  • Cell Line
  • Cloning, Molecular
  • Extracellular Signal-Regulated MAP Kinases
  • Humans
  • Mice
  • Mice, Knockout
  • Molecular Sequence Data
  • Organ Specificity
  • Protein-Serine-Threonine Kinases
  • RNA, Messenger
  • Rats
  • Ribosomal Protein S6 Kinases, 90-kDa