A histone H3 methyltransferase controls epigenetic events required for meiotic prophase.
Epigenetic modifications of histones regulate gene expression and chromatin structure. Here we show that Meisetz (meiosis-induced factor containing a PR/SET domain and zinc-finger motif) is a histone methyltransferase that is important for the progression of early meiotic prophase. Meisetz transcripts are detected only in germ cells entering meiotic prophase in female fetal gonads and in postnatal testis. Notably, Meisetz has catalytic activity for trimethylation, but not mono- or dimethylation, of lysine 4 of histone H3, and a transactivation activity that depends on its methylation activity. Mice in which the Meisetz gene is disrupted show sterility in both sexes due to severe impairment of the double-stranded break repair pathway, deficient pairing of homologous chromosomes and impaired sex body formation. In Meisetz-deficient testis, trimethylation of lysine 4 of histone H3 is attenuated and meiotic gene transcription is altered. These findings indicate that meiosis-specific epigenetic events in mammals are crucial for proper meiotic progression.
Pubmed ID: 16292313 RIS Download
Animals | Cloning, Molecular | Epigenesis, Genetic | Female | Gene Expression Regulation | Gonads | Histone-Lysine N-Methyltransferase | Histones | Male | Meiosis | Methylation | Mice | Mice, Inbred C57BL | Prophase | Protein Methyltransferases | Spermatocytes | Transcription, Genetic