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Identification of novel argonaute-associated proteins.

RNA silencing processes are guided by small RNAs known as siRNAs and microRNAs (miRNAs) . They reside in ribonucleoprotein complexes, which guide the cleavage of complementary mRNAs or affect stability and translation of partial complementary mRNAs . Argonaute (Ago) proteins are at the heart of silencing effector complexes and bind the single-stranded siRNA and miRNA . Our biochemical analysis revealed that Ago2 is present in a pre-miRNA processing complex that is able to transfer the miRNA into a target-mRNA cleaving complex. To gain insight into the function and composition of RNA silencing complexes, we purified Ago1- and Ago2-containing complexes from human cells. Several known Ago1- and/or Ago2-associated proteins including Dicer were identified, but also two novel factors, the putative RNA helicase MOV10, and the RNA recognition motif (RRM)-containing protein TNRC6B/KIAA1093. The new proteins localize, similar to Ago proteins, to mRNA-degrading cytoplasmic P bodies, and they are functionally required to mediate miRNA-guided mRNA cleavage.

Pubmed ID: 16289642


  • Meister G
  • Landthaler M
  • Peters L
  • Chen PY
  • Urlaub H
  • L├╝hrmann R
  • Tuschl T


Current biology : CB

Publication Data

December 6, 2005

Associated Grants

  • Agency: NIGMS NIH HHS, Id: R01 GM068476-01

Mesh Terms

  • Argonaute Proteins
  • Blotting, Northern
  • Blotting, Western
  • Cell Line
  • Eukaryotic Initiation Factor-2
  • Eukaryotic Initiation Factors
  • Fluorescent Antibody Technique
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Mass Spectrometry
  • MicroRNAs
  • Oligonucleotides
  • Peptide Initiation Factors
  • RNA Helicases
  • RNA, Messenger
  • RNA, Small Interfering
  • RNA-Induced Silencing Complex
  • Reverse Transcriptase Polymerase Chain Reaction
  • Ribonuclease III