RNA-associated autoantigens activate B cells by combined B cell antigen receptor/Toll-like receptor 7 engagement.
Previous studies (Leadbetter, E.A., I.R. Rifkin, A.H. Hohlbaum, B. Beaudette, M.J. Shlomchik, and A. Marshak-Rothstein. 2002. Nature. 416:603-607; Viglianti, G.A., C.M. Lau, T.M. Hanley, B.A. Miko, M.J. Shlomchik, and A. Marshak-Rothstein. 2003. Immunity. 19:837-847) established the unique capacity of DNA and DNA-associated autoantigens to activate autoreactive B cells via sequential engagement of the B cell antigen receptor (BCR) and Toll-like receptor (TLR) 9. We demonstrate that this two-receptor paradigm can be extended to the BCR/TLR7 activation of autoreactive B cells by RNA and RNA-associated autoantigens. These data implicate TLR recognition of endogenous ligands in the response to both DNA- and RNA-associated autoantigens. Importantly, the response to RNA-associated autoantigens was markedly enhanced by IFN-alpha, a cytokine strongly linked to disease progression in patients with systemic lupus erythematosus (SLE). As further evidence that TLRs play a key role in autoantibody responses in SLE, we found that autoimmune-prone mice, lacking the TLR adaptor protein MyD88, had markedly reduced chromatin, Sm, and rheumatoid factor autoantibody titers.
Pubmed ID: 16260486 RIS Download
Adaptor Proteins, Signal Transducing | Animals | Antigens, Differentiation | Autoantibodies | Autoantigens | B-Lymphocytes | Female | Hybridomas | Interferon-alpha | Lymphocyte Activation | Male | Membrane Glycoproteins | Mice | Mice, Inbred C57BL | Mice, Inbred MRL lpr | Mice, Knockout | Mice, Transgenic | Myeloid Differentiation Factor 88 | RNA | Receptors, Antigen, B-Cell | Receptors, Immunologic | Ribonucleoproteins | Toll-Like Receptor 7 | Toll-Like Receptor 9