We have updated our privacy policy. If you have any question, contact us at privacy@scicrunch.org. Dismiss and don't show again

Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Heterogeneous nuclear ribonucleoprotein C1/C2, MeCP1, and SWI/SNF form a chromatin remodeling complex at the beta-globin locus control region.

Locus control regions (LCRs) are regulatory DNA sequences that are situated many kilobases away from their cognate promoters. LCRs protect transgenes from position effect variegation and heterochromatinization and also promote copy-number dependence of the levels of transgene expression. In this work, we describe the biochemical purification of a previously undescribed LCR-associated remodeling complex (LARC) that consists of heterogeneous nuclear ribonucleoprotein C1/C2, nucleosome remodeling SWI/SNF, and nucleosome remodeling and deacetylating (NuRD)/MeCP1 as a single homogeneous complex. LARC binds to the hypersensitive 2 (HS2)-Maf recognition element (MARE) DNA in a sequence-specific manner and remodels nucleosomes. Heterogeneous nuclear ribonucleoprotein C1/C2, previously known as a general RNA binding protein, provides a sequence-specific DNA recognition element for LARC, and the LARC DNA-recognition sequence is essential for the enhancement of transcription by HS2. Independently of the initiation of transcription, LARC becomes associated with beta-like globin promoters.

Pubmed ID: 16217013 RIS Download

Mesh terms: Amino Acid Sequence | Chromosomal Proteins, Non-Histone | DNA-Binding Proteins | Globins | Heterogeneous-Nuclear Ribonucleoprotein Group C | Histone Deacetylases | Humans | K562 Cells | Locus Control Region | Molecular Sequence Data | Multiprotein Complexes | Nucleosomes | Promoter Regions, Genetic | Sequence Alignment | Transcription Factors

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NIGMS NIH HHS, Id: R01 GM048405
  • Agency: NHLBI NIH HHS, Id: N01-HV-28186
  • Agency: NIGMS NIH HHS, Id: GM048405
  • Agency: NHLBI NIH HHS, Id: N01HV28186
  • Agency: NIGMS NIH HHS, Id: R37 GM048405
  • Agency: NHLBI NIH HHS, Id: P01 HL063357
  • Agency: NHLBI NIH HHS, Id: IP01HL63357-06

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.