Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Towards a proteome-scale map of the human protein-protein interaction network.

Nature | Oct 20, 2005

Systematic mapping of protein-protein interactions, or 'interactome' mapping, was initiated in model organisms, starting with defined biological processes and then expanding to the scale of the proteome. Although far from complete, such maps have revealed global topological and dynamic features of interactome networks that relate to known biological properties, suggesting that a human interactome map will provide insight into development and disease mechanisms at a systems level. Here we describe an initial version of a proteome-scale map of human binary protein-protein interactions. Using a stringent, high-throughput yeast two-hybrid system, we tested pairwise interactions among the products of approximately 8,100 currently available Gateway-cloned open reading frames and detected approximately 2,800 interactions. This data set, called CCSB-HI1, has a verification rate of approximately 78% as revealed by an independent co-affinity purification assay, and correlates significantly with other biological attributes. The CCSB-HI1 data set increases by approximately 70% the set of available binary interactions within the tested space and reveals more than 300 new connections to over 100 disease-associated proteins. This work represents an important step towards a systematic and comprehensive human interactome project.

Pubmed ID: 16189514 RIS Download

Mesh terms: Cloning, Molecular | Humans | Open Reading Frames | Protein Binding | Proteome | RNA | Saccharomyces cerevisiae | Two-Hybrid System Techniques

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NHGRI NIH HHS, Id: RC4 HG006066
  • Agency: NHGRI NIH HHS, Id: R01 HG001715
  • Agency: NHGRI NIH HHS, Id: U01 HG001715
  • Agency: NHGRI NIH HHS, Id: P50 HG004233
  • Agency: NCI NIH HHS, Id: R33 CA132073
  • Agency: NHLBI NIH HHS, Id: U01 HL098166

BioGRID (Data, Interactions)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.