IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction.
Type I interferons are central mediators for antiviral responses. Using high-throughput functional screening of interferon inducers, we have identified here a molecule we call interferon-beta promoter stimulator 1 (IPS-1). Overexpression of IPS-1 induced type I interferon and interferon-inducible genes through activation of IRF3, IRF7 and NF-kappaB transcription factors. TBK1 and IKKi protein kinases were required for the IPS-1-mediated interferon induction. IPS-1 contained an N-terminal CARD-like structure that mediated interaction with the CARD of RIG-I and Mda5, which are cytoplasmic RNA helicases that sense viral infection. 'Knockdown' of IPS-1 by small interfering RNA blocked interferon induction by virus infection. Thus, IPS-1 is an adaptor involved in RIG-I- and Mda5-mediated antiviral immune responses.
Pubmed ID: 16127453 RIS Download
Adaptor Proteins, Signal Transducing | Amino Acid Sequence | Animals | Cells, Cultured | DEAD Box Protein 58 | DEAD-box RNA Helicases | DNA-Binding Proteins | Humans | I-kappa B Kinase | Interferon Inducers | Interferon Regulatory Factor-3 | Interferon Regulatory Factor-7 | Interferon Type I | Interferon-Induced Helicase, IFIH1 | Interferon-beta | Mice | Molecular Sequence Data | NF-kappa B | Protein Structure, Tertiary | Protein-Serine-Threonine Kinases | RNA Helicases | RNA, Messenger | Sequence Alignment | Transcription Factors | Vesicular stomatitis Indiana virus