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Reduction of macrophage infiltration and chemoattractant gene expression changes in white adipose tissue of morbidly obese subjects after surgery-induced weight loss.

Diabetes | Aug 27, 2005

http://www.ncbi.nlm.nih.gov/pubmed/16046292

In human obesity, the stroma vascular fraction (SVF) of white adipose tissue (WAT) is enriched in macrophages. These cells may contribute to low-grade inflammation and to its metabolic complications. Little is known about the effect of weight loss on macrophages and genes involved in macrophage attraction. We examined subcutaneous WAT (scWAT) of 7 lean and 17 morbidly obese subjects before and 3 months after bypass surgery. Immunomorphological changes of the number of scWAT-infiltrating macrophages were evaluated, along with concomitant changes in expression of SVF-overexpressed genes. The number of scWAT-infiltrating macrophages before surgery was higher in obese than in lean subjects (HAM56+/CD68+; 22.6 +/- 4.3 vs. 1.4 +/- 0.6%, P < 0.001). Typical "crowns" of macrophages were observed around adipocytes. Drastic weight loss resulted in a significant decrease in macrophage number (-11.63 +/- 2.3%, P < 0.001), and remaining macrophages stained positive for the anti-inflammatory protein interleukin 10. Genes involved in macrophage attraction (monocyte chemotactic protein [MCP]-1, plasminogen activator urokinase receptor [PLAUR], and colony-stimulating factor [CSF]-3) and hypoxia (hypoxia-inducible factor-1alpha [HIF-1alpha]), expression of which increases in obesity and decreases after surgery, were predominantly expressed in the SVF. We show that improvement of the inflammatory profile after weight loss is related to a reduced number of macrophages in scWAT. MCP-1, PLAUR, CSF-3, and HIF-1alpha may play roles in the attraction of macrophages in scWAT.

Pubmed ID: 16046292 RIS Download

Mesh terms: Adipose Tissue | Adult | Anastomosis, Roux-en-Y | Chemokine CCL2 | Chemotactic Factors | DNA-Binding Proteins | Female | Gastric Bypass | Gene Expression | Granulocyte Colony-Stimulating Factor | Humans | Hypoxia-Inducible Factor 1 | Hypoxia-Inducible Factor 1, alpha Subunit | Macrophages | Middle Aged | Nuclear Proteins | Obesity, Morbid | Oligonucleotide Array Sequence Analysis | Receptors, Cell Surface | Receptors, Urokinase Plasminogen Activator | Recombinant Proteins | Transcription Factors | Weight Loss

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