The genome of the kinetoplastid parasite, Leishmania major.
Leishmania species cause a spectrum of human diseases in tropical and subtropical regions of the world. We have sequenced the 36 chromosomes of the 32.8-megabase haploid genome of Leishmania major (Friedlin strain) and predict 911 RNA genes, 39 pseudogenes, and 8272 protein-coding genes, of which 36% can be ascribed a putative function. These include genes involved in host-pathogen interactions, such as proteolytic enzymes, and extensive machinery for synthesis of complex surface glycoconjugates. The organization of protein-coding genes into long, strand-specific, polycistronic clusters and lack of general transcription factors in the L. major, Trypanosoma brucei, and Trypanosoma cruzi (Tritryp) genomes suggest that the mechanisms regulating RNA polymerase II-directed transcription are distinct from those operating in other eukaryotes, although the trypanosomatids appear capable of chromatin remodeling. Abundant RNA-binding proteins are encoded in the Tritryp genomes, consistent with active posttranscriptional regulation of gene expression.
Pubmed ID: 16020728 RIS Download
Animals | Chromatin | Gene Expression Regulation | Genes, Protozoan | Genes, rRNA | Genome, Protozoan | Glycoconjugates | Leishmania major | Leishmaniasis, Cutaneous | Lipid Metabolism | Membrane Proteins | Molecular Sequence Data | Multigene Family | Protein Biosynthesis | Protein Processing, Post-Translational | Protozoan Proteins | RNA Processing, Post-Transcriptional | RNA Splicing | RNA, Protozoan | Sequence Analysis, DNA | Transcription, Genetic