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The genome of the kinetoplastid parasite, Leishmania major.

Leishmania species cause a spectrum of human diseases in tropical and subtropical regions of the world. We have sequenced the 36 chromosomes of the 32.8-megabase haploid genome of Leishmania major (Friedlin strain) and predict 911 RNA genes, 39 pseudogenes, and 8272 protein-coding genes, of which 36% can be ascribed a putative function. These include genes involved in host-pathogen interactions, such as proteolytic enzymes, and extensive machinery for synthesis of complex surface glycoconjugates. The organization of protein-coding genes into long, strand-specific, polycistronic clusters and lack of general transcription factors in the L. major, Trypanosoma brucei, and Trypanosoma cruzi (Tritryp) genomes suggest that the mechanisms regulating RNA polymerase II-directed transcription are distinct from those operating in other eukaryotes, although the trypanosomatids appear capable of chromatin remodeling. Abundant RNA-binding proteins are encoded in the Tritryp genomes, consistent with active posttranscriptional regulation of gene expression.

Pubmed ID: 16020728

Authors

  • Ivens AC
  • Peacock CS
  • Worthey EA
  • Murphy L
  • Aggarwal G
  • Berriman M
  • Sisk E
  • Rajandream MA
  • Adlem E
  • Aert R
  • Anupama A
  • Apostolou Z
  • Attipoe P
  • Bason N
  • Bauser C
  • Beck A
  • Beverley SM
  • Bianchettin G
  • Borzym K
  • Bothe G
  • Bruschi CV
  • Collins M
  • Cadag E
  • Ciarloni L
  • Clayton C
  • Coulson RM
  • Cronin A
  • Cruz AK
  • Davies RM
  • De Gaudenzi J
  • Dobson DE
  • Duesterhoeft A
  • Fazelina G
  • Fosker N
  • Frasch AC
  • Fraser A
  • Fuchs M
  • Gabel C
  • Goble A
  • Goffeau A
  • Harris D
  • Hertz-Fowler C
  • Hilbert H
  • Horn D
  • Huang Y
  • Klages S
  • Knights A
  • Kube M
  • Larke N
  • Litvin L
  • Lord A
  • Louie T
  • Marra M
  • Masuy D
  • Matthews K
  • Michaeli S
  • Mottram JC
  • Müller-Auer S
  • Munden H
  • Nelson S
  • Norbertczak H
  • Oliver K
  • O'neil S
  • Pentony M
  • Pohl TM
  • Price C
  • Purnelle B
  • Quail MA
  • Rabbinowitsch E
  • Reinhardt R
  • Rieger M
  • Rinta J
  • Robben J
  • Robertson L
  • Ruiz JC
  • Rutter S
  • Saunders D
  • Schäfer M
  • Schein J
  • Schwartz DC
  • Seeger K
  • Seyler A
  • Sharp S
  • Shin H
  • Sivam D
  • Squares R
  • Squares S
  • Tosato V
  • Vogt C
  • Volckaert G
  • Wambutt R
  • Warren T
  • Wedler H
  • Woodward J
  • Zhou S
  • Zimmermann W
  • Smith DF
  • Blackwell JM
  • Stuart KD
  • Barrell B
  • Myler PJ

Journal

Science (New York, N.Y.)

Publication Data

July 15, 2005

Associated Grants

  • Agency: NIAID NIH HHS, Id: R01 AI040599
  • Agency: NIAID NIH HHS, Id: R01 AI053667
  • Agency: NIAID NIH HHS, Id: U01 AI040599
  • Agency: Wellcome Trust, Id:

Mesh Terms

  • Animals
  • Chromatin
  • Gene Expression Regulation
  • Genes, Protozoan
  • Genes, rRNA
  • Genome, Protozoan
  • Glycoconjugates
  • Leishmania major
  • Leishmaniasis, Cutaneous
  • Lipid Metabolism
  • Membrane Proteins
  • Molecular Sequence Data
  • Multigene Family
  • Protein Biosynthesis
  • Protein Processing, Post-Translational
  • Protozoan Proteins
  • RNA Processing, Post-Transcriptional
  • RNA Splicing
  • RNA, Protozoan
  • Sequence Analysis, DNA
  • Transcription, Genetic