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Physical association and coordinate function of the H3 K4 methyltransferase MLL1 and the H4 K16 acetyltransferase MOF.

Cell | Jun 17, 2005

http://www.ncbi.nlm.nih.gov/pubmed/15960975

A stable complex containing MLL1 and MOF has been immunoaffinity purified from a human cell line that stably expresses an epitope-tagged WDR5 subunit. Stable interactions between MLL1 and MOF were confirmed by reciprocal immunoprecipitation, cosedimentation, and cotransfection analyses, and interaction sites were mapped to MLL1 C-terminal and MOF zinc finger domains. The purified complex has a robust MLL1-mediated histone methyltransferase activity that can effect mono-, di-, and trimethylation of H3 K4 and a MOF-mediated histone acetyltransferase activity that is specific for H4 K16. Importantly, both activities are required for optimal transcription activation on a chromatin template in vitro and on an endogenous MLL1 target gene, Hox a9, in vivo. These results indicate an activator-based mechanism for joint MLL1 and MOF recruitment and targeted methylation and acetylation and provide a molecular explanation for the closely correlated distribution of H3 K4 methylation and H4 K16 acetylation on active genes.

Pubmed ID: 15960975 RIS Download

Mesh terms: Acetyltransferases | DNA-Binding Proteins | HeLa Cells | Histone Acetyltransferases | Histone-Lysine N-Methyltransferase | Homeodomain Proteins | Humans | Microfilament Proteins | Multienzyme Complexes | Myeloid-Lymphoid Leukemia Protein | Proto-Oncogenes | Transcription Factors | Zinc Fingers

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BioGRID (Data, Interactions)

GO (Data, Gene Annotation)

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