Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Development of a unique system for spatiotemporal and lineage-specific gene expression in mice.

http://www.ncbi.nlm.nih.gov/pubmed/15941831

We have developed an advanced method for conditional gene expression in mice that integrates the Cre-mediated and tetracycline-dependent expression systems. An rtTA gene, preceded by a loxP-flanked STOP sequence, was inserted into the ROSA26 locus to create a R26STOPrtTA mouse strain. When the STOP sequence is excised by Cre-mediated recombination, the rtTA is expressed in the Cre-expressing cells and all of their derivatives. Therefore, cell type-, tissue-, or lineage-specific expression of rtTA is achieved by the use of an appropriate Cre transgenic strain. In mice also carrying a target gene under the control of the tetracycline response element, inducible expression of the target gene is temporally regulated by administration of doxycycline. Our results demonstrate that this universal system is uniquely suited for spatiotemporal and lineage-specific gene expression in an inducible fashion. Gene expression can be manipulated in specific cell types and lineages with a flexibility that is difficult to achieve with conventional methods.

Pubmed ID: 15941831 RIS Download

Mesh terms: Animals | Doxycycline | Gene Expression | Gene Targeting | Genetic Engineering | Green Fluorescent Proteins | Integrases | Mice | Mice, Transgenic | Neural Crest | Proteins | RNA, Untranslated | Tetracycline | Transcription Factors | beta-Galactosidase

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NCI NIH HHS, Id: CA106308
  • Agency: NIDDK NIH HHS, Id: DK55388
  • Agency: NICHD NIH HHS, Id: HD44265
  • Agency: NCI NIH HHS, Id: R01 CA106308

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.