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A missense mutation in the previously undescribed gene Tmhs underlies deafness in hurry-scurry (hscy) mice.

Mouse deafness mutations provide valuable models of human hearing disorders and entry points into molecular pathways important to the hearing process. A newly discovered mouse mutation named hurry-scurry (hscy) causes deafness and vestibular dysfunction. Scanning electron microscopy of cochleae from 8-day-old mutants revealed disorganized hair bundles, and by 50 days of age, many hair cells are missing. To positionally clone hscy, 1,160 F(2) mice were produced from an intercross of (C57BL/6-hscy x CAST/EiJ) F(1) hybrids, and the mutation was localized to a 182-kb region of chromosome 17. A missense mutation causing a critical cysteine to phenylalanine codon change was discovered in a previously undescribed gene within this candidate interval. The gene is predicted to encode an integral membrane protein with four transmembrane helices. A synthetic peptide designed from the predicted protein was used to produce specific polyclonal antibodies, and strong immunoreactivity was observed on hair bundles of both inner and outer hair cells in cochleae of newborn +/+ controls and +/hscy heterozygotes but was absent in hscy/hscy mutants. Accordingly, the gene was given the name "tetraspan membrane protein of hair cell stereocilia," symbol Tmhs. Two related proteins (>60% amino acid identity) are encoded by genes on mouse chromosomes 5 and 6 and, together with the Tmhs-encoded protein (TMHS), comprise a distinct tetraspan subfamily. Our localization of TMHS to the apical membrane of inner ear hair cells during the period of stereocilia formation suggests a function in hair bundle morphogenesis.

Pubmed ID: 15905332


  • Longo-Guess CM
  • Gagnon LH
  • Cook SA
  • Wu J
  • Zheng QY
  • Johnson KR


Proceedings of the National Academy of Sciences of the United States of America

Publication Data

May 31, 2005

Associated Grants

  • Agency: NCI NIH HHS, Id: CA34196
  • Agency: NIDCD NIH HHS, Id: DC04301
  • Agency: NIDCD NIH HHS, Id: R01 DC009246
  • Agency: NCRR NIH HHS, Id: RR01183

Mesh Terms

  • Animals
  • Base Sequence
  • Blotting, Northern
  • Chromosome Mapping
  • Cluster Analysis
  • Crosses, Genetic
  • DNA, Complementary
  • Deafness
  • Gene Components
  • Gene Expression
  • Hair Cells, Auditory
  • Histological Techniques
  • Immunohistochemistry
  • Membrane Proteins
  • Mice
  • Mice, Mutant Strains
  • Microscopy, Electron, Scanning
  • Molecular Sequence Data
  • Mutation, Missense
  • Sequence Analysis, DNA