Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Tea4p links microtubule plus ends with the formin for3p in the establishment of cell polarity.

Developmental cell | Apr 5, 2005

http://www.ncbi.nlm.nih.gov/pubmed/15809031

Microtubules regulate actin-based processes such as cell migration and cytokinesis, but molecular mechanisms are not understood. In the fission yeast Schizosaccharomyces pombe, microtubule plus ends regulate cell polarity in part by transporting the kelch repeat protein tea1p to cell ends. Here, we identify tea4p, a SH3 domain protein that binds directly to tea1p. Like tea1p, tea4p localizes to growing microtubule plus ends and to cortical sites at cell ends, and it is necessary for the establishment of bipolar growth. Tea4p binds directly to and recruits the formin for3p, which nucleates actin cable assembly. During "new end take off" (NETO), formation of a protein complex that includes tea1p, tea4p, and for3p is necessary and sufficient for the establishment of cell polarity and localized actin assembly at new cell ends. Our results suggest a molecular mechanism for how microtubule plus ends regulate the spatial distribution of actin assembly.

Pubmed ID: 15809031 RIS Download

Mesh terms: Actins | Amino Acid Sequence | Cell Cycle Proteins | Cell Polarity | Cytoskeleton | Microtubule-Associated Proteins | Microtubules | Molecular Sequence Data | Multiprotein Complexes | Recombinant Fusion Proteins | Schizosaccharomyces | Schizosaccharomyces pombe Proteins | Sequence Alignment | Two-Hybrid System Techniques | src Homology Domains

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: PHS HHS, Id: R0156836

GO (Data, Gene Annotation)

BioGRID (Data, Interactions)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.