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PARP-1 determines specificity in a retinoid signaling pathway via direct modulation of mediator.

We show that PARP-1 is indispensable to retinoic acid receptor (RAR)-mediated transcription from the RARbeta2 promoter in a highly purified, reconstituted transcription system and that RA-inducible expression of all RARbeta isoforms is abrogated in PARP-1(-/-) cells in vivo. Importantly, PARP-1 activity was independent of its catalytic domain. PARP-1 directly interacts with RAR and Mediator. Chromatin immunoprecipitation experiments confirmed the presence of PARP-1 and Mediator on RAR-responsive promoters in vivo. Importantly, Mediator was inactive (Cdk8+) under basal conditions but was activated (Cdk8-) upon induction. However, in PARP-1(-/-) cells, Mediator was retained in its inactive state (Cdk8+) upon induction consistent with the absence of gene expression. PARP-1 became dispensable for ligand-dependent transcription in a chromatin reconstituted transcription assay when Mediator was devoid of the Cdk8 module (CRSP). PARP-1 appears to function as a specificity factor regulating the RA-induced switch of Mediator from the inactive (Cdk8+) to the active (Cdk8-) state in RAR-dependent transcription.

Pubmed ID: 15808511

Authors

  • Pavri R
  • Lewis B
  • Kim TK
  • Dilworth FJ
  • Erdjument-Bromage H
  • Tempst P
  • de Murcia G
  • Evans R
  • Chambon P
  • Reinberg D

Journal

Molecular cell

Publication Data

April 1, 2005

Associated Grants

None

Mesh Terms

  • Animals
  • Cell Line
  • Cell Nucleus
  • Dimerization
  • Gene Deletion
  • HeLa Cells
  • Humans
  • Poly(ADP-ribose) Polymerases
  • Receptors, Retinoic Acid
  • Recombinant Proteins
  • Reverse Transcriptase Polymerase Chain Reaction
  • Signal Transduction
  • Transcription, Genetic
  • Transfection