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Profound block in thymocyte development in mice lacking p56lck.

Nature | May 14, 1992

http://www.ncbi.nlm.nih.gov/pubmed/1579166

The protein Lck (p56lck) has a relative molecular mass of 56,000 and belongs to the Src family of tyrosine kinases. It is expressed exclusively in lymphoid cells, predominantly in thymocytes and peripheral T cells. Lck associates specifically with the cytoplasmic domains of both CD4 and CD8 T-cell surface glycoproteins and interacts with the beta-chain of the interleukin-2 receptor, which implicates Lck activity in signal transduction during thymocyte ontogeny and activation of mature T cells. Here we generate an lck null mutation by homologous recombination in embryonic stem cells to evaluate the role of p56lck in T-cell development and activation. Lck-deficient mice show a pronounced thymic atrophy, with a dramatic reduction in the double-positive (CD4+CD8+) thymocyte population. Mature, single-positive thymocytes are not detectable in these mice and there are only very few peripheral T cells. These results illustrate the crucial role of this T-cell-specific tyrosine kinase in the thymocyte development.

Pubmed ID: 1579166 RIS Download

Mesh terms: Animals | Antisense Elements (Genetics) | B-Lymphocytes | Base Sequence | Heterozygote | Immunoglobulins | Lymphocyte Specific Protein Tyrosine Kinase p56(lck) | Mice | Mice, Mutant Strains | Molecular Sequence Data | Mutagenesis, Insertional | Oligodeoxyribonucleotides | Protein-Tyrosine Kinases | T-Lymphocyte Subsets | T-Lymphocytes

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