Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Mammalian polycomb-mediated repression of Hox genes requires the essential spliceosomal protein Sf3b1.

Genes & development | Mar 1, 2005

http://www.ncbi.nlm.nih.gov/pubmed/15741318

Polycomb group (PcG) proteins are responsible for the stable repression of homeotic (Hox) genes by forming multimeric protein complexes. We show (1) physical interaction between components of the U2 small nuclear ribonucleoprotein particle (U2 snRNP), including Sf3b1 and PcG proteins Zfp144 and Rnf2; and (2) that Sf3b1 heterozygous mice exhibit skeletal transformations concomitant with ectopic Hox expressions. These alterations are enhanced by Zfp144 mutation but repressed by Mll mutation (a trithorax-group gene). Importantly, the levels of Sf3b1 in PcG complexes were decreased in Sf3b1-heterozygous embryos. These findings suggest that Sf3b1-PcG protein interaction is essential for true PcG-mediated repression of Hox genes.

Pubmed ID: 15741318 RIS Download

Mesh terms: Animals | Bone and Bones | DNA-Binding Proteins | Down-Regulation | Gene Expression Regulation, Developmental | Histone-Lysine N-Methyltransferase | Homeodomain Proteins | Mice | Mice, Knockout | Mutation | Myeloid-Lymphoid Leukemia Protein | Phosphoproteins | Polycomb Repressive Complex 1 | Protein Binding | Proto-Oncogenes | Repressor Proteins | Ribonucleoprotein, U2 Small Nuclear | Transcription Factors | Two-Hybrid System Techniques | Ubiquitin-Protein Ligases

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

None

Mouse Genome Informatics (Data, Gene Annotation)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.