• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

The mammalian YL1 protein is a shared subunit of the TRRAP/TIP60 histone acetyltransferase and SRCAP complexes.

The multiprotein mammalian TRRAP/TIP60-containing histone acetyltransferase (HAT) complex performs critical functions in a variety of cellular processes including transcriptional activation, double strand DNA break repair, and apoptosis. We previously isolated the TRRAP/TIP60 complex from HeLa cells (Cai, Y., Jin, J., Tomomori-Sato, C., Sato, S., Sorokina, I., Parmely, T. J., Conaway, R. C., and Conaway, J. W. (2003) J. Biol. Chem. 278, 42733-42736). Analysis of proteins present in preparations of the TRRAP/TIP60 complex led to the identification of several new subunits, as well as several potential subunits including the YL1 protein. Here we present evidence that the YL1 protein is a previously unrecognized subunit of the TRRAP/TIP60 HAT complex. In addition, we present evidence that YL1 is also a component of a novel mammalian multiprotein complex that includes the SNF2-related helicase SRCAP and resembles the recently described Saccharomyces cerevisiae SWR1 chromatin remodeling complex. Taken together, our findings identify the YL1 protein as a new subunit of the TRRAP/TIP60 HAT complex, and they suggest that YL1 plays multiple roles in chromatin modification and remodeling in cells.

Pubmed ID: 15647280

Authors

  • Cai Y
  • Jin J
  • Florens L
  • Swanson SK
  • Kusch T
  • Li B
  • Workman JL
  • Washburn MP
  • Conaway RC
  • Conaway JW

Journal

The Journal of biological chemistry

Publication Data

April 8, 2005

Associated Grants

  • Agency: NIGMS NIH HHS, Id: R37 GM41628

Mesh Terms

  • Acetyltransferases
  • Adaptor Proteins, Signal Transducing
  • Adenosine Triphosphatases
  • Animals
  • DNA-Binding Proteins
  • HeLa Cells
  • Histone Acetyltransferases
  • Humans
  • Multiprotein Complexes
  • Nuclear Proteins
  • Protein Subunits
  • Repressor Proteins